Protocols shared by authors via RaP service
Journal
Lifespan assay
Authors: Jongsun Lee, Sieun S. and Seung-Jae V.
Date: Feb 2, 2021，Views：814
Original protocol used in research article：
A mutation that alters the 255th glutamate to lysine in RSKS-1/S6 kinase reliably extends the lifespan of C. elegans
microPublication Biology, Oct 27, 2020; DOI: 10.17912/micropub.biology.000322
Dissection
Authors: Vera D Rinaldi and Oliver J Rando
Date: Feb 2, 2021，Views：899
Original protocol used in research article：
An atlas of cell types in the mouse epididymis and vas deferens
eLife, Jul 30, 2020; DOI: 10.7554/eLife.55474
Gene set enrichment analysis (GSEA) of sex-biased genes
Authors: Meritxell Oliva, Barbara E. Stranger and Daniel Cotter
Date: Feb 2, 2021，Views：1024
Original protocol used in research article：
The impact of sex on gene expression across human tissues
Science, Sep 11, 2020; DOI: 10.1126/science.aba3066
Identification of sex-differential gene expression
Authors: Meritxell Oliva, Barbara E. Stranger, Manuel Muñoz-Aguirre and Yuxin Zou
Date: Feb 2, 2021，Views：1000
Original protocol used in research article：
The impact of sex on gene expression across human tissues
Science, Sep 11, 2020; DOI: 10.1126/science.aba3066
TCR analysis
Author: Paul Gueguen
Date: Feb 2, 2021，Views：893
Original protocol used in research article：
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer
Science Immunology, Jan 29, 2021; DOI: 10.1126/sciimmunol.abd5778
Structural determination and refinement
Author: Ming quan Xie
Date: Feb 2, 2021，Views：793
Original protocol used in research article：
Structural insights into the signal transduction mechanism of the K+-sensing two-component system KdpDE
Science Signaling, Aug 3, 2020; DOI: 10.1126/scisignal.aaz2970
Modulation of RBC cholesterol
Author: Rima S Chakrabarti
Date: Feb 1, 2021，Views：846
Original protocol used in research article：
Variability of cholesterol accessibility in human red blood cells measured using a bacterial cholesterol-binding toxin
eLife, Feb 8, 2017; DOI: 10.7554/eLife.23355
Association between the Wuhan city travel ban and the arrival time of COVID-19 in other cities
Authors: Yonghong Liu and Chieh-Hsi Wu
Date: Feb 1, 2021，Views：1008
Original protocol used in research article：
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
Science, May 7, 2020; DOI: 10.1126/science.abb6105
Fc.IL-2 muteins
Authors: Daniel J. Campbell and Marc A. Gavin
Date: Feb 1, 2021，Views：950
Original protocol used in research article：
An IL-2 mutein engineered to promote expansion of regulatory T cells arrests ongoing autoimmunity in mice
Science Immunology, Aug 13, 2020; DOI: 10.1126/sciimmunol.aba5264
Ion transport activity assay of microbial rhodopsin expressed in E. coli cells
Authors: Masae Konno, Keiichi Inoue and Hideki Kandori
Date: Jan 31, 2021，Views：5035
Original protocol used in research article：
Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps
Science Advances, Apr 9, 2020; DOI: 10.1126/sciadv.aaz2441
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