Protocols shared by authors via RaP service
Journal
Muscle myoblast isolation and culture
Author: Shihuan Kuang
Date: Feb 15, 2021，Views：943
Original protocol used in research article：
Stage-specific effects of Notch activation during skeletal myogenesis
eLife, Sep 19, 2016; DOI: 10.7554/eLife.17355
Multiplex FISH in CaRMA-imaged tissue after in vivo calcium imaging
Authors: Hui Yang, Shengjin Xu and Scott M. Sternson
Date: Feb 15, 2021，Views：988
Original protocol used in research article：
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494
Alignment of the GRIN lens
Authors: Zhongya Qin, Nancy Y. Ip and Jianan Y. Qu
Date: Feb 15, 2021，Views：1008
Original protocol used in research article：
Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
Science Advances, Sep 29, 2020; DOI: 10.1126/sciadv.abc6521
HCN4 immunostaining in human (Alomone labs antibody)
Authors: Angélique Bordey and Lena Nguyen
Date: Feb 13, 2021，Views：828
Original protocol used in research article：
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI: 10.1126/scitranslmed.abc1492
Migration assay
Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 12, 2021，Views：929
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Generation of in vitro trained TAMs
Authors: Poonam Ashwin Kumar, Andreas Weigert and Rajkumar Savai
Date: Feb 12, 2021，Views：941
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Article inclusion
Author: Alyson Haslam
Date: Feb 12, 2021，Views：1066
Original protocol used in research article：
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183
Data analysis
Author: Alyson Haslam
Date: Feb 12, 2021，Views：1050
Original protocol used in research article：
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183
Flow cytometry
Authors: Takuya Sekine, André Perez-Potti and Marcus Buggert
Date: Feb 11, 2021，Views：978
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Clinical Trial Design: Summary, Recipient Eligibility Criteria, Donor Selection Criteria, Donor/Recipient Seromatching, and Study Conduct
Author: Diwakar Davar
Date: Feb 11, 2021，Views：965
Original protocol used in research article：
Fecal microbiota transplant overcomes resistance to anti–PD-1 therapy in melanoma patients
Science, Feb 5, 2021; DOI: 10.1126/science.abf3363
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