Bio-101 - Improve Research Reproducibility

Dimensionality reduction and clustering

Authors: Dingbang Ma, Dariusz Przybylski, Katharine Abruzzi, Matthias Schlichting, Qunlong Li, Xi Long and Michael Rosbash
Date: Feb 11, 2021，Views：1087

Original protocol used in research article：

A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056

Single-cell RNA library preparation

Authors: Dingbang Ma, Dariusz Przybylski, Katharine Abruzzi, Matthias Schlichting, Qunlong Li, Xi Long and Michael Rosbash
Date: Feb 11, 2021，Views：1130

Original protocol used in research article：

A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056

Protocol of full article

Author: Shakil Ahmed
Date: Feb 10, 2021，Views：918

Original protocol used in research article：

Pyrogallol induces oxidative stress defects in the fission yeast S. pombe
microPublication Biology, Jan 7, 2021; DOI: 10.17912/micropub.biology.000348

Measurement of cellular or lysosomal iron levels

Authors: King Faisal Yambire and Nuno Raimundo
Date: Feb 10, 2021，Views：1085

Original protocol used in research article：

Impaired lysosomal acidification triggers iron deficiency and inflammation in vivo
eLife, Dec 3, 2019; DOI: 10.7554/eLife.51031

fRIP

Author: Alexis S Bailey
Date: Feb 8, 2021，Views：1035

Original protocol used in research article：

The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
eLife, Oct 31, 2017; DOI: 10.7554/eLife.26116

Flow cytometry data analysis

Authors: Christopher Maucourant, Iva Filipovic, Andrea Ponzetta and Niklas K. Björkström
Date: Feb 8, 2021，Views：1302

Original protocol used in research article：

Natural killer cell immunotypes related to COVID-19 disease severity
Science Immunology, Aug 21, 2020; DOI: 10.1126/sciimmunol.abd6832

Estimation of MTD

Authors: Natasha Vinod, Chad V. Pecot and Alexander V. Kabanov
Date: Feb 7, 2021，Views：888

Original protocol used in research article：

High-capacity poly(2-oxazoline) formulation of TLR 7/8 agonist extends survival in a chemo-insensitive metastatic model of lung adenocarcinoma
Science Advances, Jun 16, 2020; DOI: 10.1126/sciadv.aba5542

Yeast strain construction and transformation

Authors: Deze Kong and Christina D. Smolke
Date: Feb 7, 2021，Views：1002

Original protocol used in research article：

Discovery of a previously unknown biosynthetic capacity of naringenin chalcone synthase by heterologous expression of a tomato gene cluster in yeast
Science Advances, Oct 29, 2020; DOI: 10.1126/sciadv.abd1143

Cell death, membrane depolarization, and membrane fluidity quantification assays

Author: Julia Bos
Date: Feb 5, 2021，Views：850

Original protocol used in research article：

Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure
Science Advances, Jan 20, 2021; DOI: 10.1126/sciadv.abd1033

CRISPR/Cas9 Flag knockin

Authors: Joana G Marques and Beat W Schäfer
Date: Feb 5, 2021，Views：1099

Original protocol used in research article：

NuRD subunit CHD4 regulates super-enhancer accessibility in rhabdomyosarcoma and represents a general tumor dependency
eLife, Aug 3, 2020; DOI: 10.7554/eLife.54993

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