Protocols shared by authors via RaP service
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List of antibodies, siRNAs and shRNAs
Author: Seyed Mehdi Jafarnejad
Date: Feb 24, 2021，Views：881
Original protocol used in research article：
Translational control of ERK signaling through miRNA/4EHP-directed silencing
eLife, Feb 7, 2018; DOI: 10.7554/eLife.35034
RNA extraction from individual zebrafish embryos
Authors: Neha Wali, Richard White and Elisabeth M Busch-Nentwich
Date: Feb 24, 2021，Views：1221
Original protocol used in research article：
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
Cell culture, infection and vitrification
Authors: Georg Wolff and Montserrat Bárcena
Date: Feb 24, 2021，Views：1141
Original protocol used in research article：
A molecular pore spans the double membrane of the coronavirus replication organelle
Science, Aug 6, 2020; DOI: 10.1126/science.abd3629
Measurement of thickness of the inner mucus layer
Authors: Takahide Ara, Daigo Hashimoto and Takanori Teshima
Date: Feb 24, 2021，Views：923
Original protocol used in research article：
Intestinal goblet cells protect against GVHD after allogeneic stem cell transplantation via Lypd8
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaw0720
Task implementations
Author: Jin Fan
Date: Feb 24, 2021，Views：918
Original protocol used in research article：
Anterior insular cortex plays a critical role in interoceptive attention
eLife, Apr 15, 2019; DOI: 10.7554/eLife.42265
Detection of proteins in membrane fractions
Authors: Alam García-Heredia, Takehiro Kado, Caralyn E. Sein, Julia Puffal, Sarah H. Osman, Julius Judd, Todd A. Gray, Yasu S. Morita and M. Sloan Siegrist
Date: Feb 23, 2021，Views：953
Original protocol used in research article：
Membrane-partitioned cell wall synthesis in mycobacteria
eLife, Feb 5, 2021; DOI: 10.7554/eLife.60263
Protocol of full article
Author: Kenneth Trimmer
Date: Feb 23, 2021，Views：795
Original protocol used in research article：
CRISPR-edit point mutant allele detection (CEPAD)-PCR method for rapid screening of CRISPR edited point mutations
microPublication Biology, Jan 1, 1970; DOI: 10.17912/micropub.biology.000368
Subcellular fractionation
Authors: Tyler B Faust and Alan D Frankel
Date: Feb 22, 2021，Views：881
Original protocol used in research article：
The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI: 10.7554/eLife.31879
Sequences, plasmids, transfection and RNA interference
Author: Zhou Yu
Date: Feb 22, 2021，Views：858
Original protocol used in research article：
Lys29-linkage of ASK1 by Skp1−Cullin 1−Fbxo21 ubiquitin ligase complex is required for antiviral innate response
eLife, Apr 11, 2016; DOI: 10.7554/eLife.14087
Immune signature, immune infiltration, and nontumor fraction
Authors: Yue Wang and Da Yang
Date: Feb 22, 2021，Views：858
Original protocol used in research article：
LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abb3555
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