Protocols shared by authors via RaP service
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Matrigel Invasion Assay Protocol
Authors: Chen Dong, Pooja Popli, Ramakrishna Kommagani and Thorold W Theunissen
Date: Jan 28, 2021，Views：8276
Original protocol used in research article：
Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504
X-ray crystallographic analysis of the WT erα LBD-BZA complex
Author: Sean W Fanning
Date: Jan 28, 2021，Views：826
Original protocol used in research article：
The SERM/SERD bazedoxifene disrupts ESR1 helix 12 to overcome acquired hormone resistance in breast cancer cells
eLife, Nov 29, 2018; DOI: 10.7554/eLife.37161
Synthesis and functionalization of AgNPs
Author: Ludovico Cantuti-Castelvetri
Date: Jan 28, 2021，Views：974
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
In vitro pull down and Co-IP assays
Authors: Claudia Castillo-González and Xiuren Zhang
Date: Jan 28, 2021，Views：2628
Original protocol used in research article：
Geminivirus-encoded TrAP suppressor inhibits the histone methyltransferase SUVH4/KYP to counter host defense
eLife, Sep 7, 2015; DOI: 10.7554/eLife.06671
Animals, human tissue, and mouse cells
Author: Khaled S. Abd-Elrahman
Date: Jan 28, 2021，Views：787
Original protocol used in research article：
Aβ oligomers induce pathophysiological mGluR5 signaling in Alzheimer’s disease model mice in a sex-selective manner
Science Signaling, Dec 14, 2020; DOI: 10.1126/scisignal.abd2494
Western blot assay
Authors: Leiming Wang, Sophia Y. Tsai and Ming-Jer Tsai
Date: Jan 28, 2021，Views：993
Original protocol used in research article：
Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8031
Electrophysiology
Author: Wallace B Thoreson
Date: Jan 27, 2021，Views：864
Original protocol used in research article：
Ca2+ sensor synaptotagmin-1 mediates exocytosis in mammalian photoreceptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.45946
Barcode to gRNA assignment
Authors: Ryan Muller and Nicholas T. Ingolia
Date: Jan 27, 2021，Views：861
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Cryo-EM structural analysis of Ad5-3M
Author: Phoebe L. Stewart
Date: Jan 27, 2021，Views：940
Original protocol used in research article：
Systemic cancer therapy with engineered adenovirus that evades innate immunity
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.abc6659
Creating acetic acid-induced artifical gastric ulcers in rats
Authors: Philip Wai-Yan Chiu, Liming Bian and Xiayi Xu
Date: Jan 27, 2021，Views：874
Original protocol used in research article：
Bioadhesive hydrogels demonstrating pH-independent and ultrafast gelation promote gastric ulcer healing in pigs
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aba8014
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