Protocols shared by authors via RaP service
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Antibodies, IP, and Western blot
Author: Shashank Srivastava
Date: Feb 18, 2021，Views：902
Original protocol used in research article：
NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia
Science Advances, Jan 26, 2021; DOI: 10.1126/sciadv.abc9781
RNA decay
Author: Ariel Alejandro Bazzini
Date: Feb 18, 2021，Views：1048
Original protocol used in research article：
Translation affects mRNA stability in a codon-dependent manner in human cells
eLife, Apr 23, 2019; DOI: 10.7554/eLife.45396
Isolation of adult mouse and rat cardiomyocytes
Authors: Huiliang Zhang and Peter S Rabinovitch
Date: Feb 17, 2021，Views：1368
Original protocol used in research article：
Reduction of elevated proton leak rejuvenates mitochondria in the aged cardiomyocyte
eLife, Dec 15, 2020; DOI: 10.7554/eLife.60827
Quantifying γδT-cell activation in vivo and in vitro
Authors: Krithika Badarinath, Ravindra Zirmire, Colin Jamora and Isha Rana
Date: Feb 17, 2021，Views：1155
Original protocol used in research article：
Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
eLife, Dec 4, 2017; DOI: 10.7554/eLife.28875
Mating assays
Author: Patricia J Wittkopp
Date: Feb 17, 2021，Views：870
Original protocol used in research article：
The yellow gene influences Drosophila male mating success through sex comb melanization
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49388
VivosX assay for MCF10A Cells
Authors: Lisa M Kim and Cheuk T Leung
Date: Feb 17, 2021，Views：871
Original protocol used in research article：
VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells
eLife, Aug 9, 2018; DOI: 10.7554/eLife.36654
MACS sorting of TAMs from human and mouse lung tumor tissues
Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 17, 2021，Views：876
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
mRNAs half-lives determination
Author: Ning Dai
Date: Feb 16, 2021，Views：866
Original protocol used in research article：
IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
eLife, Jul 28, 2017; DOI: 10.7554/eLife.27155
Protein expression, purification, and site-specific labeling
Author: Jesper Levring
Date: Feb 16, 2021，Views：1003
Original protocol used in research article：
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451
Cloning of mlCNBD-FRET
Author: Dagmar Wachten
Date: Feb 16, 2021，Views：991
Original protocol used in research article：
A novel biosensor to study cAMP dynamics in cilia and flagella
eLife, Mar 22, 2016; DOI: 10.7554/eLife.14052
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