Protocols shared by authors via RaP service
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AAV vectors and stereotactic microinjections
Author: Esther Visser
Date: Feb 5, 2021，Views：798
Original protocol used in research article：
A persistent alcohol cue memory trace drives relapse to alcohol seeking after prolonged abstinence
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aax7060
Co-expression analysis
Authors: Shuqing Xu, Ian T. Baldwin and Jiancai Li
Date: Feb 4, 2021，Views：966
Original protocol used in research article：
Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity
Science, Jan 15, 2021; DOI: 10.1126/science.abe4713
Fluctuation assays
Author: R Blake Billmyre
Date: Feb 4, 2021，Views：944
Original protocol used in research article：
Natural mismatch repair mutations mediate phenotypic diversity and drug resistance in Cryptococcus deuterogattii
eLife, Sep 26, 2017; DOI: 10.7554/eLife.28802
Detailed reprogramming protocol
Author: Avani Yeola
Date: Feb 4, 2021，Views：881
Original protocol used in research article：
Induction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine
Science Advances, Jan 13, 2021; DOI: 10.1126/sciadv.abd1929
Animal Study
Author: Thomas F. Rogers
Date: Feb 4, 2021，Views：905
Original protocol used in research article：
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Science, Aug 21, 2020; DOI: 10.1126/science.abc7520
Exploratory bifactor analysis
Authors: Christopher Gagne and Sonia J Bishop
Date: Feb 4, 2021，Views：989
Original protocol used in research article：
Impaired adaptation of learning to contingency volatility in internalizing psychopathology
eLife, Dec 22, 2020; DOI: 10.7554/eLife.61387
SUMOylation and in vitro SUMOylation assay
Author: Xiancai Ma
Date: Feb 4, 2021，Views：874
Original protocol used in research article：
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42426
Assessment of thrombolysis efficacy in a mouse model
Authors: Yu Gao and Wang si yu
Date: Feb 3, 2021，Views：859
Original protocol used in research article：
Accelerating thrombolysis using a precision and clot-penetrating drug delivery strategy by nanoparticle-shelled microbubbles
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aaz8204
Immunohistochemistry protocol for analyzing Hedgehog pathway factors in mouse embryos
Authors: Saishu Yoshida and Kiyotsugu Yoshida
Date: Feb 3, 2021，Views：903
Original protocol used in research article：
The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis
eLife, Aug 6, 2020; DOI: 10.7554/eLife.57381
tRNA charging assay
Authors: Natalya N Pavlova and Craig B Thompson
Date: Feb 3, 2021，Views：1701
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
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