Protocols shared by authors via RaP service
Journal
Western blotting
Authors: Sthitapranjya Pati and Vidita A Vaidya
Date: Mar 1, 2022，Views：762
Original protocol used in research article：
Chronic postnatal chemogenetic activation of forebrain excitatory neurons evokes persistent changes in mood behavior
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56171
Microchannel migration imaging
Authors: Scott B Thompson and Jordan Jacobelli
Date: Feb 24, 2022，Views：671
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
RNA interference
Authors: Shawn C. Chafe and Shoukat Dedhar
Date: Feb 24, 2022，Views：669
Original protocol used in research article：
Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors
Science Advances, Aug 26, 2021; DOI: 10.1126/sciadv.abj0364
2.2. Nanoemulsion Preparation
Authors: Mandana Lotfi and Ali Akbar Moghadamnia
Date: Feb 23, 2022，Views：636
Original protocol used in research article：
Protective Effect of Quercetin Nanoemulsion on 5-Fluorouracil-Induced Oral Mucositis in Mice
Journal of Oncology, Jun 9, 2021; DOI: 10.1155/2021/5598230
Experimental tumor dormancy
Author: Masoud H. Manjili
Date: Feb 23, 2022，Views：618
Original protocol used in research article：
Autophagy-deficient breast cancer shows early tumor recurrence and escape from dormancy
Oncotarget, Apr 24, 2018; DOI: 10.18632/oncotarget.25197
Genome-wide association studies
Authors: Ella Katz and Daniel J Kliebenstein
Date: Feb 23, 2022，Views：715
Original protocol used in research article：
Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe
eLife, May 5, 2021; DOI: 10.7554/eLife.67784
Protocol of full article
Authors: Ryan Rahman and Richard H. Gomer
Date: Feb 23, 2022，Views：677
Original protocol used in research article：
Annotating Putative D. discoideum Proteins Using I-TASSER
microPublication Biology, ; DOI: 10.17912/micropub.biology.000420
Immunohistochemistry analysis
Authors: Hyuk-Kwon Kwon and Francis Y. Lee
Date: Feb 23, 2022，Views：643
Original protocol used in research article：
Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis
Science Advances, Jun 24, 2021; DOI: 10.1126/sciadv.abf2665
Admixture-aware scan for signatures of local adaptation
Authors: Stephanie M Yan and Rajiv C McCoy
Date: Feb 23, 2022，Views：704
Original protocol used in research article：
Local adaptation and archaic introgression shape global diversity at human structural variant loci
eLife, Sep 16, 2021; DOI: 10.7554/eLife.67615
Study design
Authors: Thanaphong Phongpreecha and Thomas J. Montine
Date: Feb 23, 2022，Views：678
Original protocol used in research article：
Single-synapse analyses of Alzheimer’s disease implicate pathologic tau, DJ1, CD47, and ApoE
Science Advances, Dec 15, 2021; DOI: 10.1126/sciadv.abk0473
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