Protocols shared by authors via RaP service
Journal
Histology
Authors: Nichola N Barron and David A Bechtold
Date: Feb 12, 2022，Views：937
Original protocol used in research article：
Adipocyte NR1D1 dictates adipose tissue expansion during obesity
eLife, Aug 5, 2021; DOI: 10.7554/eLife.63324
IIH6 Assays
Authors: Ameya Walimbe and Kevin P Campbell
Date: Feb 10, 2022，Views：809
Original protocol used in research article：
The functional O-mannose glycan on α-dystroglycan contains a phospho-ribitol primed for matriglycan addition
eLife, Apr 29, 2016; DOI: 10.7554/eLife.14473
Radiochemistry of [11C]MK-6884 and [3H]MK-6884
Author: Wenping Li
Date: Feb 7, 2022，Views：780
Original protocol used in research article：
The PET tracer [11C]MK-6884 quantifies M4 muscarinic receptor in rhesus monkeys and patients with Alzheimer’s disease
Science Translational Medicine, Jan 12, 2022; DOI: 10.1126/scitranslmed.abg3684
Spatial analysis of cell clusters
Authors: Nicholas JP Ryba and Lars J von Buchholtz
Date: Feb 7, 2022，Views：739
Original protocol used in research article：
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752
Phagocytosis assays
Author: Rasmus Berglund
Date: Feb 7, 2022，Views：717
Original protocol used in research article：
Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077
RNA-Seq
Authors: Prashant Rajbhandari and Peter Tontonoz
Date: Feb 4, 2022，Views：790
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Antibodies
Authors: Ji Eun Oh and Akiko Iwasaki
Date: Feb 2, 2022，Views：722
Original protocol used in research article：
Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA
Science Immunology, Dec 10, 2021; DOI: 10.1126/sciimmunol.abj5129
Enzyme-linked immunosorbent assay
Authors: Ji Eun Oh and Akiko Iwasaki
Date: Feb 2, 2022，Views：830
Original protocol used in research article：
Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA
Science Immunology, Dec 10, 2021; DOI: 10.1126/sciimmunol.abj5129
Computational-driven epitope verification and affinity maturation of TLR4-targeting antibodies
Author: Bilal Ahmad
Date: Feb 2, 2022，Views：727
Original protocol used in research article：
Computational-Driven Epitope Verification and Affinity Maturation of TLR4-Targeting Antibodies
International Journal of Molecular Sciences, Jun 1, 2021; DOI: 10.3390/ijms22115989
Tumor model generation and treatment
Authors: Leila Akkari and Johanna A. Joyce
Date: Feb 2, 2022，Views：761
Original protocol used in research article：
Dynamic changes in glioma macrophage populations after radiotherapy reveal CSF-1R inhibition as a strategy to overcome resistance
Science Translational Medicine, Jul 14, 2020; DOI: 10.1126/scitranslmed.aaw7843
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