Protocols shared by authors via RaP service
Journal
Slingshot
Authors: Bryan Marsh and Robert Blelloch
Date: Mar 24, 2022，Views：703
Original protocol used in research article：
Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266
Generation of E. coli strains
Authors: Shar-yin N. Huang, Nadim Majdalani and Yves Pommier
Date: Mar 22, 2022，Views：701
Original protocol used in research article：
Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
Science Advances, Mar 3, 2021; DOI: 10.1126/sciadv.abe0384
Rotarod
Authors: Erik C. Böttger and David Paul Wolfer
Date: Mar 22, 2022，Views：679
Original protocol used in research article：
Premature aging in mice with error-prone protein synthesis
Science Advances, Mar 2, 2022; DOI: 10.1126/sciadv.abl9051
Ubiquitination assay
Authors: Di Feng and Bo Gao
Date: Mar 22, 2022，Views：3006
Original protocol used in research article：
Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7–mediated Vangl ubiquitination and endoplasmic reticulum–associated degradation
Science Advances, May 14, 2021; DOI: 10.1126/sciadv.abg2099
CRISPR knockout and siRNA knockdown
Authors: Zhiping Weng and Kevin Struhl
Date: Mar 22, 2022，Views：769
Original protocol used in research article：
YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation
eLife, Aug 31, 2021; DOI: 10.7554/eLife.67312
Culture conditions
Authors: Milo S Johnson and Michael M Desai
Date: Mar 21, 2022，Views：744
Original protocol used in research article：
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations
eLife, Jan 19, 2021; DOI: 10.7554/eLife.63910
Supervised classification
Authors: Diptendu Mukherjee, Eyal Itskovits and Ami Citri
Date: Mar 21, 2022，Views：661
Original protocol used in research article：
Salient experiences are represented by unique transcriptional signatures in the mouse brain
eLife, Feb 7, 2018; DOI: 10.7554/eLife.31220
Expression and preparation of proteins
Authors: Tao Xie, Tamjeed Saleh, Paolo Rossi and Charalampos G. Kalodimos
Date: Mar 21, 2022，Views：685
Original protocol used in research article：
Conformational states dynamically populated by a kinase determine its function
Science, Oct 8, 2020; DOI: 10.1126/science.abc2754
Preparation for two-photon calcium imaging in visual cortex
Authors: Carsen Stringer, Marius Pachitariu and Kenneth D. Harris
Date: Mar 18, 2022，Views：624
Original protocol used in research article：
Spontaneous behaviors drive multidimensional, brainwide activity
Science, Apr 19, 2019; DOI: 10.1126/science.aav7893
Generation of erythroid-specific TLR9-deficient mice
Authors: LK Metthew Lam, Kaitlyn Eckart and Nilam S. Mangalmurti
Date: Mar 17, 2022，Views：762
Original protocol used in research article：
DNA binding to TLR9 expressed by red blood cells promotes innate immune activation and anemia
Science Translational Medicine, Oct 20, 2021; DOI: 10.1126/scitranslmed.abj1008
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