Protocols shared by authors via RaP service
Journal
Protein analysis
Author: Shadmehr Demehri
Date: Apr 13, 2022，Views：633
Original protocol used in research article：
CD8+ T cell immunity blocks the metastasis of carcinogen-exposed breast cancer
Science Advances, Jun 17, 2021; DOI: 10.1126/sciadv.abd8936
Clustering of PVH neuron functional responses measured by CaRMA imaging
Authors: Shengjin Xu, Hui Yang and Scott Sternson
Date: Apr 11, 2022，Views：646
Original protocol used in research article：
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494
Methods
Authors: Jiacan Su, Mingyao Liu and Jian Luo
Date: Apr 11, 2022，Views：590
Original protocol used in research article：
Regulation of body length and bone mass by Gpr126/Adgrg6
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aaz0368
Comparative metabolic profiling
Authors: Jin-Ming Yang and Jianxun Song
Date: Apr 8, 2022，Views：582
Original protocol used in research article：
Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8+ T cells
Science Advances, Feb 2, 2022; DOI: 10.1126/sciadv.abl9783
The CSiN score and the other neoantigen-based metrics used for comparison
Author: Tao Wang
Date: Apr 8, 2022，Views：590
Original protocol used in research article：
Tumor neoantigenicity assessment with CSiN score incorporates clonality and immunogenicity to predict immunotherapy outcomes
Science Immunology, Feb 20, 2020; DOI: 10.1126/sciimmunol.aaz3199
Cell culture
Authors: Xiaohao Liu, Fenyong Sun, Yu Yang, Xiaoli Zhu and Weihong Tan
Date: Apr 8, 2022，Views：647
Original protocol used in research article：
Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging
Science Advances, Jan 14, 2022; DOI: 10.1126/sciadv.abk0133
Computational model
Authors: Satoshi Toda, Wendell A. Lim and Teemu J. Hakkinen
Date: Apr 7, 2022，Views：656
Original protocol used in research article：
Engineering synthetic morphogen systems that can program multicellular patterning
Science, Oct 15, 2020; DOI: 10.1126/science.abc0033
Lentiviral
Authors: Kotryna Vaidziulyte, Kristine Schauer and Mathieu Coppey
Date: Apr 6, 2022，Views：797
Original protocol used in research article：
Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking
eLife, Mar 18, 2022; DOI: 10.7554/eLife.69229
Northern tRNA charging assay
Authors: Natalya N Pavlova and Craig B Thompson
Date: Apr 1, 2022，Views：715
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Histone extraction and derivatization
Authors: Sydney P Thomas and John M Denu
Date: Apr 1, 2022，Views：756
Original protocol used in research article：
Short-chain fatty acids activate acetyltransferase p300
eLife, Oct 22, 2021; DOI: 10.7554/eLife.72171
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