Protocols shared by authors via RaP service
Journal
Construction of the FICA system
Authors: Meiyan Wang and Haifeng Ye
Date: May 3, 2022，Views：624
Original protocol used in research article：
A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation
Science Advances, Dec 10, 2021; DOI: 10.1126/sciadv.abh2358
Immunohistochemistry of murine retina
Authors: Mervin C. Yoder, Maria B. Grant and Sergio Li Calzi
Date: May 2, 2022，Views：637
Original protocol used in research article：
Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice
Science Advances, Mar 4, 2022; DOI: 10.1126/sciadv.abm5559
Dot blot assay
Authors: Yong-gui Wu and Xiao-ming Meng
Date: Apr 29, 2022，Views：791
Original protocol used in research article：
Inhibition of METTL3 attenuates renal injury and inflammation by alleviating TAB3 m6A modifications via IGF2BP2-dependent mechanisms
Science Translational Medicine, Apr 13, 2022; DOI: 10.1126/scitranslmed.abk2709
IMC data
Authors: James N Arnold and Shahram Kordasti
Date: Apr 28, 2022，Views：655
Original protocol used in research article：
ImmunoCluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry data
eLife, Apr 30, 2021; DOI: 10.7554/eLife.62915
Isolation and cryopreservation of gonads
Authors: Michael J McGrew and Jun Hu
Date: Apr 28, 2022，Views：660
Original protocol used in research article：
A low-tech, cost-effective and efficient method for safeguarding genetic diversity by direct cryopreservation of poultry embryonic reproductive cells
eLife, Jan 25, 2022; DOI: 10.7554/eLife.74036
JAGuaR Alignment
Author: Richard Corbett
Date: Apr 26, 2022，Views：590
Original protocol used in research article：
A Scalable Strand-Specific Protocol Enabling Full-Length Total RNA Sequencing From Single Cells
Frontiers in Genetics, Jun 3, 2021; DOI: 10.3389/fgene.2021.665888
Preparation of bovine serum albumin (BSA)-conjugated palmitic acid
Author: Fei Hua
Date: Apr 26, 2022，Views：910
Original protocol used in research article：
Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway
BMC Nephrology, Jun 10, 2021; DOI: 10.1186/s12882-021-02391-1
Proliferation assay and cell cycle profiling via flow cytometry
Author: Li Lan
Date: Apr 26, 2022，Views：614
Original protocol used in research article：
cGAS suppresses genomic instability as a decelerator of replication forks
Science Advances, Oct 13, 2020; DOI: 10.1126/sciadv.abb8941
Immunohistochemistry
Author: Sungjin Park
Date: Apr 25, 2022，Views：611
Original protocol used in research article：
The release of surface-anchored α-tectorin an apical extracellular matrix protein mediates tectorial membrane organization
Science Advances, Nov 26, 2019; DOI: 10.1126/sciadv.aay6300
Coevolutionary analysis and coarse-grained simulations
Authors: Francesco Colizzi and Modesto Orozco
Date: Apr 25, 2022，Views：608
Original protocol used in research article：
Probing allosteric regulations with coevolution-driven molecular simulations
Science Advances, Sep 8, 2021; DOI: 10.1126/sciadv.abj0786
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