Protocols shared by authors via RaP service
Journal
Senescence-associated β-galactosidase assay
Authors: Mounir El Maï and Miguel Godinho Ferreira
Date: Mar 7, 2022，Views：889
Original protocol used in research article：
Opposing p53 and mTOR/AKT promote an in vivo switch from apoptosis to senescence upon telomere shortening in zebrafish
eLife, May 19, 2020; DOI: 10.7554/eLife.54935
Crown delineation
Author: Ben G Weinstein
Date: Mar 7, 2022，Views：682
Original protocol used in research article：
A remote sensing derived data set of 100 million individual tree crowns for the National Ecological Observatory Network
eLife, Feb 19, 2021; DOI: 10.7554/eLife.62922
2.2.2. Maceration Extraction (ME)
Authors: Jurga Andreja Kazlauskaite and Jurga Bernatoniene
Date: Mar 6, 2022，Views：587
Original protocol used in research article：
Novel Extraction Method Using Excipients to Enhance Yield of Genistein and Daidzein in Trifolium pratensis L.
Pharmaceutics, May 22, 2021; DOI: 10.3390/pharmaceutics13060777
Drug preparation
Author: Lu Chen
Date: Mar 6, 2022，Views：723
Original protocol used in research article：
Defective memory engram reactivation underlies impaired fear memory recall in Fragile X syndrome
eLife, Nov 20, 2020; DOI: 10.7554/eLife.61882
Phytaspase cleavage assay using synthetic oligopeptide substrates
Authors: A. Stintzi and A. Schaller
Date: Mar 4, 2022，Views：649
Original protocol used in research article：
Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI: 10.1126/science.aaz5641
Identification of individual potentially tumor-reactive T cells
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：781
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Indexes characterizing meta-clusters and STARTRAC analysis
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：921
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Immunohistochemistry
Authors: Jose Felix Moruno Manchon and Andrey S Tsvetkov
Date: Mar 3, 2022，Views：655
Original protocol used in research article：
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI: 10.7554/eLife.52283
Cell cultures and transfection
Authors: Jose Felix Moruno Manchon and Andrey S Tsvetkov
Date: Mar 3, 2022，Views：644
Original protocol used in research article：
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI: 10.7554/eLife.52283
DiI tracing
Authors: Nicolas Renier and Alain Chédotal
Date: Mar 3, 2022，Views：646
Original protocol used in research article：
A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
eLife, Mar 28, 2017; DOI: 10.7554/eLife.23494
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