Protocols shared by authors via RaP service
Journal
Luciferase reporter assay
Authors: Munir R Tanas and Keith Garcia
Date: May 25, 2022，Views：2696
Original protocol used in research article：
TAZ-CAMTA1 and YAP-TFE3 alter the TAZ/YAP transcriptome by recruiting the ATAC histone acetyltransferase complex
eLife, Apr 29, 2021; DOI: 10.7554/eLife.62857
Organoid viability assay
Authors: Hans R Schöler and Jan M Bruder
Date: May 25, 2022，Views：735
Original protocol used in research article：
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids
eLife, Nov 3, 2020; DOI: 10.7554/eLife.52904
Spectral decomposition
Author: Magnus Andersson
Date: May 21, 2022，Views：598
Original protocol used in research article：
Tracking Ca2+ ATPase intermediates in real time by x-ray solution scattering
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aaz0981
Purification of IgG specific to full-length VAR2CSA ectodomains
Authors: Justin Y. A. Doritchamou and Patrick E Duffy
Date: May 18, 2022，Views：732
Original protocol used in research article：
A single full-length VAR2CSA ectodomain variant purifies broadly neutralizing antibodies against placental malaria isolates
eLife, Feb 1, 2022; DOI: 10.7554/eLife.76264
Plants, growth conditions, and field plantations
Authors: Danny Kessler and Ian T Baldwin
Date: May 17, 2022，Views：680
Original protocol used in research article：
How scent and nectar influence floral antagonists and mutualists
eLife, Jul 1, 2015; DOI: 10.7554/eLife.07641
AIF subcellular localization analysis and determination of AIF pathology score
Authors: Stephen P. Chelko and Nazareno Paolocci
Date: May 16, 2022，Views：610
Original protocol used in research article：
Exercise triggers CAPN1-mediated AIF truncation inducing myocyte cell death in arrhythmogenic cardiomyopathy
Science Translational Medicine, Feb 16, 2021; DOI: 10.1126/scitranslmed.abf0891
Membrane stability index (MSI)
Author: Natalie De Falco
Date: May 16, 2022，Views：718
Original protocol used in research article：
Geodiversity impacts plant community structure in a semi-arid region
Scientific Reports, Jul 27, 2021; DOI: 10.1038/s41598-021-94698-0
Thermal seizure induction
Author: Daniel K Mulkey
Date: May 13, 2022，Views：629
Original protocol used in research article：
Disordered breathing in a mouse model of Dravet syndrome
eLife, Apr 26, 2019; DOI: 10.7554/eLife.43387
Single-cell isolations
Author: Jonathan Kipnis
Date: May 11, 2022，Views：682
Original protocol used in research article：
Skull and vertebral bone marrow are myeloid cell reservoirs for the meninges and CNS parenchyma
Science, Jul 23, 2021; DOI: 10.1126/science.abf7844
Study design
Authors: Christian Schwartz and Padraic G. Fallon
Date: May 10, 2022，Views：581
Original protocol used in research article：
Innate PD-L1 limits T cell–mediated adipose tissue inflammation and ameliorates diet-induced obesity
Science Translational Medicine, Mar 9, 2022; DOI: 10.1126/scitranslmed.abj6879
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