Protocols shared by authors via RaP service
Journal
Establishing Endometrial Assembloids to Model Human Embryo Implantation in vitro
Authors: Thomas M Rawlings, Maria Tryfonos, Komal Makwana, Deborah M Taylor, Jan J Brosens and Emma S Lucas
Date: Jun 15, 2022，Views：894
Original protocol used in research article：
Modelling the impact of decidual senescence on embryo implantation in human endometrial assembloids
eLife, Sep 6, 2021; DOI: 10.7554/eLife.69603
The Therapeutic Factors Inventory-19 (TFI-19)
Authors: Nicholas K. Canby and Kristina Eichel
Date: Jun 14, 2022，Views：683
Original protocol used in research article：
The Contribution of Common and Specific Therapeutic Factors to Mindfulness-Based Intervention Outcomes
Frontiers in Psychology, Jan 14, 2021; DOI: 10.3389/fpsyg.2020.603394
Urine-based Enzyme-Linked Immunosorbent Assay to detect anti-SARS-CoV-2 nucleocapsid protein antibodies
Authors: Fernanda Ludolf, Fernanda F Ramos, Flávia Bagno, Eduardo A F Coelho and Flavio G Fonseca
Date: Jun 14, 2022，Views：767
Original protocol used in research article：
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion
Science Advances, May 13, 2022; DOI: 10.1126/sciadv.abn7424
Ribosome profiling with retapamulin treatment (Ribo-RET)
Authors: Keith M Derbyshire, Todd A Gray and Joseph T Wade
Date: Jun 13, 2022，Views：617
Original protocol used in research article：
Pervasive translation in Mycobacterium tuberculosis
eLife, ; DOI: 10.7554/eLife.73980
Plasmids and lentivirus production
Authors: Diljeet Gill, Inês Milagre and Wolf Reik
Date: Jun 13, 2022，Views：893
Original protocol used in research article：
Multi-omic rejuvenation of human cells by maturation phase transient reprogramming
eLife, Apr 8, 2022; DOI: 10.7554/eLife.71624
Acridine orange/ethidium bromide (AO/EtBr) double staining
Authors: Ahmet Hamdi KEPEKÇİ and Cenk KIG
Date: Jun 13, 2022，Views：1954
Original protocol used in research article：
In Vitro Physiological Effects of Betahistine on Cell Lines of Various Origins
Turkish Journal of Pharmaceutical Sciences, Apr 20, 2021; DOI: 10.4274/tjps.galenos.2020.88155
Single-molecule fluorescence in situ hybridisation (smFISH)
Authors: Jeffrey Y Lee, Peter AC Wing, Alfredo Castello, Jane A Mckeating and Ilan Davis
Date: Jun 13, 2022，Views：654
Original protocol used in research article：
Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences
eLife, Jan 20, 2022; DOI: 10.7554/eLife.74153
Mitochondrial OXPHOS efficiency and capacity
Authors: Jeffrey E Pessin and Yan Tang
Date: Jun 12, 2022，Views：564
Original protocol used in research article：
TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife, Mar 7, 2022; DOI: 10.7554/eLife.73360
Lentivirus infection
Author: Edward R Kastenhuber
Date: Jun 9, 2022，Views：629
Original protocol used in research article：
Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry
eLife, Mar 16, 2022; DOI: 10.7554/eLife.77444
Lipid content measurement
Authors: Pan Su, Qiang Wang and Qing Yi
Date: Jun 9, 2022，Views：561
Original protocol used in research article：
Enhanced lipid accumulation and metabolism are required for the differentiation and activation of tumor-associated macrophages
Cancer Res, Feb 3, 2020; DOI: 10.1158/0008-5472.CAN-19-2994
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