Protocols shared by authors via RaP service
Journal
LC-MS analysis of free and esterified fatty acids.
Authors: Qing Yi and Liuling Xiao
Date: Jun 22, 2022，Views：561
Original protocol used in research article：
IL-9/STAT3/fatty acid oxidation–mediated lipid peroxidation contributes to Tc9 cell longevity and enhanced antitumor activity
The Journal of Clinical Investigation, Apr 1, 2022; DOI: 10.1172/JCI153247
HeatMap
Author: Michael Henderson
Date: Jun 22, 2022，Views：571
Original protocol used in research article：
Computational modeling of tau pathology spread reveals patterns of regional vulnerability and the impact of a genetic risk factor
Science Advances, Jun 9, 2021; DOI: 10.1126/sciadv.abg6677
IBS severity scoring system (IBS-SSS)
Authors: Laura Prospero, Giuseppe Riezzo and Francesco Russo
Date: Jun 20, 2022，Views：1493
Original protocol used in research article：
Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity
BMC Gastroenterology, May 22, 2021; DOI: 10.1186/s12876-021-01820-7
Image processing and feature extraction
Author: Anne E Carpenter
Date: Jun 20, 2022，Views：615
Original protocol used in research article：
Systematic morphological profiling of human gene and allele function via Cell Painting
eLife, Mar 18, 2017; DOI: 10.7554/eLife.24060
Cell culture, RNAi experiments, and adenoviral infection
Authors: Zhisheng Xu and Zhenji Gan
Date: Jun 20, 2022，Views：559
Original protocol used in research article：
Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength
Nature Communications, Feb 16, 2022; DOI: 10.1038/s41467-022-28557-5
OPP-click
Authors: Madeline Cassani and Geraldine SEYDOUX
Date: Jun 18, 2022，Views：630
Original protocol used in research article：
Puromycin reactivity does not accurately localize translation at the subcellular level
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60303
Immunofluorescence and analyses of PB number and ER-PB colocalization
Authors: Jason E. Lee and Gia K. Voeltz
Date: Jun 18, 2022，Views：584
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
In vivo cell depletion
Authors: Michiyuki Matsuda and Hiroshi Ichise
Date: Jun 18, 2022，Views：599
Original protocol used in research article：
Functional visualization of NK cell-mediated killing of metastatic single tumor cells
eLife, Feb 3, 2022; DOI: 10.7554/eLife.76269
Detection of SARS-CoV-2 specific IgG and IgM antibodies
Authors: Ling Wang and Zhi Q. Yao
Date: Jun 17, 2022，Views：548
Original protocol used in research article：
Blockade of SARS-CoV-2 spike protein-mediated cell–cell fusion using COVID-19 convalescent plasma
Scientific Reports, Mar 10, 2021; DOI: 10.1038/s41598-021-84840-3
A2AR expression, purification, and nanodisc reconstitution
Authors: Shuya Kate Huang and R Scott Prosser
Date: Jun 15, 2022，Views：733
Original protocol used in research article：
Allosteric modulation of the adenosine A2A receptor by cholesterol
eLife, Jan 5, 2022; DOI: 10.7554/eLife.73901
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