Protocols shared by authors via RaP service
Journal
Puromycin Immunofluorescence
Authors: Benjamin D Hobson and Peter A Sims
Date: Sep 14, 2022，Views：608
Original protocol used in research article：
Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048
Foetal cardiomyocyte size assessment
Authors: Lucas C Pantaleão, Isabella Inzani and Susan E Ozanne
Date: Sep 14, 2022，Views：564
Original protocol used in research article：
Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078
Limiting dilution transplantation
Authors: Syn Kok Yeo and Jun-Lin Guan
Date: Sep 14, 2022，Views：548
Original protocol used in research article：
Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer
eLife, Aug 25, 2020; DOI: 10.7554/eLife.58810
Chromatin immunoprecipitation (ChIP)
Authors: Alexander N Malyavko and Olga A Dontsova
Date: Sep 14, 2022，Views：593
Original protocol used in research article：
Telomere length regulation by Rif1 protein from Hansenula polymorpha
eLife, Feb 7, 2022; DOI: 10.7554/eLife.75010
Coimmunoprecipitation and immunoblot
Author: Guoliang Cui
Date: Sep 14, 2022，Views：537
Original protocol used in research article：
Rgs16 promotes antitumor CD8+ T cell exhaustion
Science Immunology, May 27, 2022; DOI: 10.1126/sciimmunol.abh1873
Western blots
Author: Junyu Xu
Date: Sep 14, 2022，Views：557
Original protocol used in research article：
KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization
eLife, Feb 9, 2022; DOI: 10.7554/eLife.72483
Immunohistochemistry
Authors: Alex Reichenbach and Zane B Andrews
Date: Sep 13, 2022，Views：555
Original protocol used in research article：
Metabolic sensing in AgRP neurons integrates homeostatic state with dopamine signalling in the striatum
eLife, Jan 12, 2022; DOI: 10.7554/eLife.72668
Protoplast Transformation of Rice Blast Fungus
Authors: Jessie Fernandez and Kim Orth
Date: Sep 13, 2022，Views：677
Original protocol used in research article：
Role of Two Metacaspases in Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
mBio, Feb 9, 2021; DOI: 10.1128/mBio.03471-20
Total RNA extraction and quantitative RT-PCR
Authors: Yan Tang and Jeffrey Pessin
Date: Sep 13, 2022，Views：616
Original protocol used in research article：
TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife, Mar 7, 2022; DOI: 10.7554/eLife.73360
Phylogenetics
Authors: Adrián A. Davín, Philip Hugenholtz, Gergely J. Szöllősi and Tom A. Williams
Date: Sep 13, 2022，Views：579
Original protocol used in research article：
A rooted phylogeny resolves early bacterial evolution
Science, May 7, 2021; DOI: 10.1126/science.abe0511
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