Bio-101 - Improve Research Reproducibility

Immunofluorescence

Authors: Kristine Schauer, Mathieu Coppey and Kotryna Vaidziulyte
Date: Sep 20, 2022，Views：548

Original protocol used in research article：

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking
eLife, Mar 18, 2022; DOI: 10.7554/eLife.69229

Primary hair cell culture

Authors: Louise Menendez, Justin Ichida and Neil Segil
Date: Sep 19, 2022，Views：564

Original protocol used in research article：

Generation of inner ear hair cells by direct lineage conversion of primary somatic cells
eLife, Jun 30, 2020; DOI: 10.7554/eLife.55249

Immuno-fluorescent (IF) staining

Authors: Minyan Zheng and Veronica F Hinman
Date: Sep 19, 2022，Views：565

Original protocol used in research article：

Regeneration of the larval sea star nervous system by wounding induced respecification to the Sox2 lineage
eLife, Jan 14, 2022; DOI: 10.7554/eLife.72983

Exploration classifier details

Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：569

Original protocol used in research article：

BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314

Exploration classifier details

Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：581

Original protocol used in research article：

BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314

DNA extraction and 16S amplicon sequencing

Authors: Steven L. Taylor and Geraint B. Rogers
Date: Sep 18, 2022，Views：554

Original protocol used in research article：

Ear microbiota and middle ear disease: a longitudinal pilot study of Aboriginal children in a remote south Australian setting
BMC Microbiology, Jan 13, 2022; DOI: 10.1186/s12866-022-02436-x

TcGDT1-3xHA overexpression

Authors: Srinivasan Ramakrishnan and Roberto Docampo
Date: Sep 17, 2022，Views：563

Original protocol used in research article：

Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn2+ in protein glycosylation needed for host cell invasion and intracellular replication
PLoS Pathogens, Mar 15, 2021; DOI: 10.1371/journal.ppat.1009399

Glucose (2-NBDG) uptake assay

Authors: Arpankumar Choksi and Samit Chattopadhyay
Date: Sep 17, 2022，Views：1094

Original protocol used in research article：

Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
Cancer & Metabolism, Apr 16, 2021; DOI: 10.1186/s40170-021-00252-x

Sporulation Test

Authors: Victor Chinomso Ujor, Venkat Gopalan and Thaddeus Chukwuemeka Ezeji
Date: Sep 16, 2022，Views：602

Original protocol used in research article：

Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant in Clostridium beijerinckii
Frontiers in Bioengineering and Biotechnology, Jun 8, 2021; DOI: 10.3389/fbioe.2021.669462

Plaque Reduction Neutralization Test (PRNT)

Authors: Sai Luo, Tom Kirchhausen, Bing Chen, Barton F. Haynes and Frederick W. Alt
Date: Sep 16, 2022，Views：586

Original protocol used in research article：

An Antibody from Single Human VH-rearranging Mouse Neutralizes All SARS-CoV-2 Variants Through BA.5 by Inhibiting Membrane Fusion
Science Immunology, Aug 11, 2022; DOI: 10.1126/sciimmunol.add5446

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