Protocols shared by authors via RaP service
Journal
Lentivirus infection of organoids to test infection efficiency
Authors: Dawei Sun and Emma L Rawlins
Date: Sep 26, 2022，Views：718
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
LC-MS/MS sample preparation
Authors: Jonathan G Van Vranken and Steven P Gygi
Date: Sep 26, 2022，Views：637
Original protocol used in research article：
Assessing target engagement using proteome-wide solvent shift assays
eLife, Dec 8, 2021; DOI: 10.7554/eLife.70784
In vitro pull-down assay
Authors: Yue Xiong and Yan-ping Xu
Date: Sep 25, 2022，Views：566
Original protocol used in research article：
USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2
Science Advances, Sep 17, 2020; DOI: 10.1126/sciadv.abc9730
Competitive fitness assays
Authors: Hala Tamim El Jarkass and Aaron W Reinke
Date: Sep 23, 2022，Views：549
Original protocol used in research article：
An intestinally secreted host factor promotes microsporidia invasion of C. elegans
eLife, Jan 7, 2022; DOI: 10.7554/eLife.72458
ASC Speck Quantification and High Magnification Imaging
Authors: Hayley I. Muendlein and Alexander Poltorak
Date: Sep 22, 2022，Views：543
Original protocol used in research article：
cFLIPL protects macrophages from LPS-induced pyroptosis via inhibition of complex II formation
Science, Mar 19, 2020; DOI: 10.1126/science.aay3878
Fabrication of ORMs and catalase conjugation
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：547
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
Synthesis and characterization of ROS-scavenging hydrogel
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：560
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
Flow cytometry–based ICAM1-binding assay
Authors: Kristoffer H. Johansen, Klaus Okkenhaug and Pamela L. Schwartzberg
Date: Sep 21, 2022，Views：651
Original protocol used in research article：
A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells
Science Signaling, Jul 19, 2022; DOI: 10.1126/scisignal.abl9169
Supporting materials for "Standardized and reproducible measurement of decision-making in mice"
Author: International Brain Laboratory
Date: Sep 21, 2022，Views：621
Original protocol used in research article：
Standardized and reproducible measurement of decision-making in mice
eLife, May 20, 2021; DOI: 10.7554/eLife.63711
Cell adhesion assay
Authors: Bochuan Li, Ying Yang, Hongfeng Jiang and Degang Liang
Date: Sep 20, 2022，Views：679
Original protocol used in research article：
RNA N6-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice
eLife, Jan 10, 2022; DOI: 10.7554/eLife.69906
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