Protocols shared by authors via RaP service
Journal
Virus Infections
Authors: Ludovico Cantuti-Castelvetri, Tambet Teesalu, Giuseppe Balistreri and Mikael Simons
Date: Oct 29, 2022，Views：419
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
Production of SARS-CoV-2 S-pseudotyped lentiviral particles
Authors: Ludovico Cantuti-Castelvetri, Tambet Teesalu, Giuseppe Balistreri and Mikael Simons
Date: Oct 29, 2022，Views：467
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
Preparing paraffin sections of human iPSC-derived kidney organoids
Author: Rafael Kramann
Date: Oct 29, 2022，Views：384
Original protocol used in research article：
SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
Cell Stem Cell, Feb 3, 2022; DOI: 10.1016/j.stem.2021.12.010
Protocol for imaging the same Class IV neurons at different stages of development
Authors: Sonal Shree and Jonathon Howard
Date: Oct 27, 2022，Views：334
Original protocol used in research article：
Dynamic instability of dendrite tips generates the highly branched morphologies of sensory neurons
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn0080
Endocytosis assay
Authors: Rawad Hodeify and Khaled Machaca
Date: Oct 27, 2022，Views：414
Original protocol used in research article：
The CCT chaperonin is a novel regulator of Ca signaling through modulation of Orai1 trafficking
Science Advances, Sep 26, 2018; DOI: 10.1126/sciadv.aau1935
Placement of torsion tracking marks
Authors: Carl D Holmgren and Jason ND Kerr
Date: Oct 26, 2022，Views：473
Original protocol used in research article：
Visual pursuit behavior in mice maintains the pursued prey on the retinal region with least optic flow
eLife, Oct 26, 2021; DOI: 10.7554/eLife.70838
m6A-RNA immunoprecipitation (MeRIP) and MeRIP-qPCR
Authors: Zhigang Hu and Zhigang Guo
Date: Oct 24, 2022，Views：506
Original protocol used in research article：
METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis
eLife, May 3, 2022; DOI: 10.7554/eLife.75231
Mass spectrometry sample preparation
Authors: Tonia L Vincent and Yoanna Ariosa-Morejon
Date: Oct 23, 2022，Views：291
Original protocol used in research article：
Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
eLife, Sep 28, 2021; DOI: 10.7554/eLife.66635
Hydroxyl radical-mediated cleavage of nucleosomal DNA
Authors: George A. Kassavetis, Grisel Cruz-Becerra and James Takuro Kadonaga
Date: Oct 22, 2022，Views：470
Original protocol used in research article：
The tardigrade damage suppressor protein binds to nucleosomes and protects DNA from hydroxyl radicals
eLife, Oct 1, 2019; DOI: 10.7554/eLife.47682
Optogenetic inhibition/activation of ERαvlVMH → DRN neurons
Authors: Pingwen Xu and Yong Xu
Date: Oct 21, 2022，Views：301
Original protocol used in research article：
An estrogen-sensitive hypothalamus-midbrain neural circuit controls thermogenesis and physical activity
Science Advances, Jan 19, 2022; DOI: 10.1126/sciadv.abk0185
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