Protocols shared by authors via RaP service
Journal
Immunofluorescence staining
Authors: Shijie Liu and James F. Martin
Date: Oct 5, 2022，Views：395
Original protocol used in research article：
Gene therapy knockdown of Hippo signaling induces cardiomyocyte renewal in pigs after myocardial infarction
Science Translational Medicine, Jun 30, 2021; DOI: 10.1126/scitranslmed.abd6892
Design and preparation of crRNA
Authors: Fuchang Yu and Longxian Zhang
Date: Oct 5, 2022，Views：322
Original protocol used in research article：
CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples
Parasites & Vectors, Apr 20, 2021; DOI: 10.1186/s13071-021-04709-2
Glutathione Peroxidase Activity Assay
Authors: Yafang Zhang and Huike Yang
Date: Oct 3, 2022，Views：378
Original protocol used in research article：
Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes
Frontiers in Cell and Developmental Biology, Mar 12, 2021; DOI: 10.3389/fcell.2021.651434
Mouse ileal organoid lines
Author: Brendan Dolan
Date: Oct 3, 2022，Views：330
Original protocol used in research article：
Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport
Science Signaling, Sep 20, 2022; DOI: 10.1126/scisignal.abl5848
Antitumor efficacy of CAR-Ts in a mouse xenograft model
Authors: Qian Xiao and Xiaolei Su
Date: Sep 29, 2022，Views：534
Original protocol used in research article：
Size-dependent activation of CAR-T cells
Science Immunology, Aug 5, 2022; DOI: 10.1126/sciimmunol.abl3995
Digital number encoding network
Authors: Gabrielle J Gutierrez and Sophie Denève
Date: Sep 29, 2022，Views：548
Original protocol used in research article：
Population adaptation in efficient balanced networks
eLife, Sep 24, 2019; DOI: 10.7554/eLife.46926
spGFP11::2HA::Ort nucleotide sequence
Authors: Tzu-Yang Lin and Chi-Hon Lee
Date: Sep 28, 2022，Views：526
Original protocol used in research article：
Antagonistic regulation by insulin-like peptide and activin ensures the elaboration of appropriate dendritic field sizes of amacrine neurons
eLife, Mar 16, 2020; DOI: 10.7554/eLife.50568
BN-PAGE
Authors: Nak Hyun Kim, Zhen-Ming Pei, Li Wan and Jeffery L. Dangl
Date: Sep 27, 2022，Views：579
Original protocol used in research article：
Plant “helper” immune receptors are Ca2+-permeable nonselective cation channels
Science, Jul 23, 2021; DOI: 10.1126/science.abg7917
Designing and cloning of the gRNA
Authors: Kumarasamypet M Mohankumar and Nithin Sam R
Date: Sep 27, 2022，Views：598
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
Preparation of single-cell suspensions
Author: Claire E. O’Leary
Date: Sep 26, 2022，Views：540
Original protocol used in research article：
Bile acid–sensitive tuft cells regulate biliary neutrophil influx
Science Immunology, Mar 4, 2022; DOI: 10.1126/sciimmunol.abj1080
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