Protocols shared by authors via RaP service
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Ex vivo digestion of B-mEVs, H-mEVs, and liposomes
Authors: Lingjun Tong, Sitong Zhang, Huaxi Yi and Jiong-Wei Wang
Date: Sep 20, 2023，Views：77
Original protocol used in research article：
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041
ELISA for TGFB1 quantification
Authors: Deepak A Kaji and Alice H Huang
Date: Sep 17, 2023，Views：68
Original protocol used in research article：
Tgfβ signaling is required for tenocyte recruitment and functional neonatal tendon regeneration
eLife, Jun 5, 2020; DOI: 10.7554/eLife.51779
Induced egress assay
Authors: Sunil Kumar Dogga and Dominique Soldati-Favre
Date: Sep 15, 2023，Views：77
Original protocol used in research article：
A druggable secretory protein maturase of Toxoplasma essential for invasion and egress
eLife, Sep 12, 2017; DOI: 10.7554/eLife.27480
EdU assay zebrafish embryos
Authors: Giulia LM Boezio, Christian SM Helker and Didier YR Stainier
Date: Sep 15, 2023，Views：276
Original protocol used in research article：
Endothelial TGF-β signaling instructs smooth muscle cell development in the cardiac outflow tract
eLife, Sep 29, 2020; DOI: 10.7554/eLife.57603
CRISPR-Cas9 knock-in of CDK12 G731E or G731R alleles
Author: Ting Han
Date: Sep 13, 2023，Views：86
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Production of rabies viral vectors
Author: Yoav Ben-Simon
Date: Sep 7, 2023，Views：212
Original protocol used in research article：
Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling
eLife, ; DOI: 10.7554/eLife.79848
T cell–mediated tumor cell killing assay
Authors: Changsheng Huang and Guihua Wang
Date: Sep 7, 2023，Views：52
Original protocol used in research article：
PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillance
Science Advances, May 26, 2023; DOI: 10.1126/sciadv.ade4186
Cell lines and cell culture
Author: changsheng huang
Date: Sep 7, 2023，Views：20
Original protocol used in research article：
PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillance
Science Advances, May 26, 2023; DOI: 10.1126/sciadv.ade4186
BG4 RIP-seq using adult mouse forebrain tissues
Authors: Sefan Asamitsu, Yasushi Yabuki and Norifumi Shioda
Date: Sep 7, 2023，Views：209
Original protocol used in research article：
RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons
Science Advances, Feb 24, 2023; DOI: 10.1126/sciadv.ade2035
Volumetric immunostaining and analysis of sympathetic nerves in tumors
Authors: Aeson Chang and Erica K. Sloan
Date: Sep 5, 2023，Views：128
Original protocol used in research article：
Beta-blockade enhances anthracycline control of metastasis in triple-negative breast cancer
Science Translational Medicine, Apr 26, 2023; DOI: 10.1126/scitranslmed.adf1147
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