Protocols shared by authors via RaP service
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Single cell isolation and flow cytometry for scRNA-Seq from utricular sensory epithelia
Authors: Mirko Scheibinger, Stefan Heller and Peter G Barr-Gillespie
Date: Dec 17, 2019，Views：1178
Original protocol used in research article：
Single-cell proteomics reveals changes in expression during hair-cell development
eLife, Nov 4, 2019; DOI: 10.7554/eLife.50777
Intact protein mass spectrometry
Author: Michael A Marletta
Date: Dec 17, 2019，Views：1789
Original protocol used in research article：
Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy
eLife, Sep 30, 2019; DOI: 10.7554/eLife.50634
Natural transformation assay
Author: Melanie Blokesch
Date: Dec 17, 2019，Views：1259
Original protocol used in research article：
Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae
eLife, Sep 3, 2019; DOI: 10.7554/eLife.48212
Fluorometry of solubilized cell extracts
Author: Seksiri Arttamangkul
Date: Dec 16, 2019，Views：1026
Original protocol used in research article：
Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
eLife, Oct 7, 2019; DOI: 10.7554/eLife.49319
Live-cell imaging
Author: Elçin Ünal
Date: Dec 16, 2019，Views：1127
Original protocol used in research article：
Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast
eLife, Aug 9, 2019; DOI: 10.7554/eLife.47156
Quantification of neural crest contributions to the ventricular myocardium and regeneration
Author: Marianne E Bronner
Date: Dec 14, 2019，Views：1333
Original protocol used in research article：
Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
eLife, Aug 8, 2019; DOI: 10.7554/eLife.47929
Flow cytometry blood staining protocol for BLT humanized mice
Author: Collin Kieffer
Date: Dec 13, 2019，Views：1260
Original protocol used in research article：
Mechanisms of virus dissemination in bone marrow of HIV-1–infected humanized BLT mice
eLife, Oct 28, 2019; DOI: 10.7554/eLife.46916
Droplet capture of single nuclei, cDNA sequencing and data analysis
Author: Nicholas Ryba
Date: Dec 13, 2019，Views：1591
Original protocol used in research article：
Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
eLife, Oct 8, 2019; DOI: 10.7554/eLife.49679
Generation of transgenic fish and chemical treatment
Author: Bo Zhang
Date: Dec 13, 2019，Views：1094
Original protocol used in research article：
One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish
eLife, Oct 30, 2019; DOI: 10.7554/eLife.48081
Metabolite extraction and LC-MS analysis
Author: Eugene I Shakhnovich
Date: Dec 12, 2019，Views：1139
Original protocol used in research article：
Adaptation to mutational inactivation of an essential gene converges to an accessible suboptimal fitness peak
eLife, Oct 1, 2019; DOI: 10.7554/eLife.50509
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