Protocols shared by authors via RaP service
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Construction of the RapiDeg yeast strain
Author: Lu Zhu
Date: Feb 26, 2020，Views：1150
Original protocol used in research article：
ESCRTs function directly on the lysosome membrane to downregulate ubiquitinated lysosomal membrane proteins
eLife, Jun 29, 2017; DOI: 10.7554/eLife.26403
Bayesian inference of progenitor types
Authors: Alfredo Llorca and Oscar Marin
Date: Feb 26, 2020，Views：1112
Original protocol used in research article：
A stochastic framework of neurogenesis underlies the assembly of neocortical cytoarchitecture
eLife, Nov 18, 2019; DOI: 10.7554/eLife.51381
Colony formation assay
Author: Ralph Patrick
Date: Feb 25, 2020，Views：4290
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
Genome Editing
Author: Grant Parker Flowers
Date: Feb 25, 2020，Views：1208
Original protocol used in research article：
Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls
eLife, Jan 28, 2020; DOI: 10.7554/eLife.48511
Single cell isolation from SVF
Author: Prashant Rajbhandari
Date: Feb 25, 2020，Views：1432
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Spike phase-coupling code
Authors: Hugo Balleza-Tapia and André Fisahn
Date: Feb 25, 2020，Views：1188
Original protocol used in research article：
TrpV1 receptor activation rescues neuronal function and network gamma oscillations from Aβ-induced impairment in mouse hippocampus in vitro
eLife, Nov 12, 2018; DOI: 10.7554/eLife.37703
Adipocyte nuclei isolation from iWAT
Author: Prashant Rajbhandari
Date: Feb 24, 2020，Views：2090
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Assembly of stalled yeast Pol II transcription elongation complex
Authors: Zhijie Chen, Ronen Gabizon, Tingting Yao and Carlos Bustamante
Date: Feb 23, 2020，Views：1406
Original protocol used in research article：
High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier
eLife, Jul 31, 2019; DOI: 10.7554/eLife.48281
Subcellular fractionation
Author: Rosa Puertollano
Date: Feb 21, 2020，Views：1321
Original protocol used in research article：
The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage
eLife, Dec 6, 2018; DOI: 10.7554/eLife.40856
Primary NSC Culture
Authors: Isabelle Maria Blomfield and François Guillemot
Date: Feb 21, 2020，Views：1327
Original protocol used in research article：
Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48561
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