Protocols shared by authors via RaP service
Journal
Measurement of acetyl-CoA
Author: Pamela Maher
Date: Mar 17, 2020，Views：1445
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Isolation and cell culture of primary alveolar macrophages
Authors: Einar Kleinhans Krogsaeter, Barbara Spix, Elisabeth Butz, Anna Scotto Rosato and Christian Grimm
Date: Mar 17, 2020，Views：1756
Original protocol used in research article：
Selective agonist of TRPML2 reveals direct role in chemokine release from innate immune cells
eLife, Nov 27, 2018; DOI: 10.7554/eLife.39720
Integration method (with replacement of go omissions)
Author: Frederick Verbruggen
Date: Mar 17, 2020，Views：1649
Original protocol used in research article：
A consensus guide to capturing the ability to inhibit actions and impulsive behaviors in the stop-signal task
eLife, Apr 29, 2019; DOI: 10.7554/eLife.46323
Immunofluorescence
Author: Nicolas Damond
Date: Mar 17, 2020，Views：1064
Original protocol used in research article：
Blockade of glucagon signaling prevents or reverses diabetes onset only if residual β-cells persist
eLife, Apr 19, 2016; DOI: 10.7554/eLife.13828
Estimation of the phase and amplitude envelope
Authors: Jessica K Nadalin and Mark A Kramer
Date: Mar 16, 2020，Views：1339
Original protocol used in research article：
A statistical framework to assess cross-frequency coupling while accounting for confounding analysis effects
eLife, Oct 16, 2019; DOI: 10.7554/eLife.44287
NPM1-NeonGreen
Author: C. Zechner
Date: Mar 13, 2020，Views：889
Original protocol used in research article：
Phase separation provides a mechanism to reduce noise in cells
Science, Jan 23, 2020; DOI: 10.1126/science.aav6691
Western blot
Authors: Valerio Azzimato and Myriam Aouadi
Date: Mar 13, 2020，Views：1268
Original protocol used in research article：
Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance
Science Translational Medicine, Feb 25, 2020; DOI: 10.1126/scitranslmed.aaw9709
Isolation and methylation profiling of cfDNA
Authors: Huiyan Luo and Rui-hua Xu
Date: Mar 12, 2020，Views：1091
Original protocol used in research article：
Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533
Protocol for preparing demembranated sperm nuclei
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：959
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
Protocol for making cycling egg extracts from Xenopus laevis
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：1062
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
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