Protocols shared by authors via RaP service
Journal
Whole mount immunostaining
Author: Manju Sharma
Date: Apr 13, 2020，Views：1265
Original protocol used in research article：
Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF
eLife, May 31, 2019; DOI: 10.7554/eLife.43352
Cell surface ELISA
Author: Amanda N Hayward
Date: Apr 13, 2020，Views：1435
Original protocol used in research article：
A toolkit for studying cell surface shedding of diverse transmembrane receptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.46983
RNP assembly for HDR reaction
Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1212
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Expression and purification of azido-Cas9 mutants
Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1256
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Bacterial culturing
Authors: Lina Yao and A Sloan Devlin
Date: Apr 11, 2020，Views：1152
Original protocol used in research article：
A selective gut bacterial bile salt hydrolase alters host metabolism
eLife, Jul 17, 2018; DOI: 10.7554/eLife.37182
Docking, pharmacophore modeling, virtual screening, and selection of test compounds
Authors: Teresa Kaserer and Daniela Schuster
Date: Apr 11, 2020，Views：1724
Original protocol used in research article：
Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876
Identification of methylation markers discriminating between CRC and normal blood
Authors: Huiyan Luo and Rui-hua Xu
Date: Apr 10, 2020，Views：988
Original protocol used in research article：
Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533
Imaging mass cytometry staining - fresh frozen human brain tissue sections
Authors: Valeria Ramaglia and Salma Sheikh-Mohamed
Date: Apr 7, 2020，Views：1959
Original protocol used in research article：
Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry
eLife, Aug 1, 2019; DOI: 10.7554/eLife.48051
Benign human prostate specimens
Authors: Chee Wai Chua and Michael M Shen
Date: Apr 7, 2020，Views：1084
Original protocol used in research article：
Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation
eLife, Jan 15, 2018; DOI: 10.7554/eLife.28768
Hydrogen deuterium exchange mass spectrometry (HDX-MS)
Authors: Euan Pyle, Zainab Ahdash, Ian Collinson and Argyris Politis
Date: Apr 4, 2020，Views：1340
Original protocol used in research article：
HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
eLife, Jul 10, 2019; DOI: 10.7554/eLife.47402
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.