Protocols shared by authors via RaP service
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Gene ontology analysis, expression analysis and SNP functional annotation
Authors: Fan Liu and Ziyi Xiong
Date: Jan 13, 2020，Views：1542
Original protocol used in research article：
Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898
iDISCO+ processing and light-sheet microscopy
Author: Nicolas Renier
Date: Jan 9, 2020，Views：1253
Original protocol used in research article：
A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
eLife, Mar 28, 2017; DOI: 10.7554/eLife.23494
Organotypic hippocampal slice cultures – electrophysiology and random-access two-photon voltage imaging
Author: Katalin Toth
Date: Jan 9, 2020，Views：1397
Original protocol used in research article：
Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators
eLife, Jul 27, 2017; DOI: 10.7554/eLife.25690
Quantitative image analysis
Author: Charlotte Kirchhelle
Date: Jan 9, 2020，Views：1076
Original protocol used in research article：
Two mechanisms regulate directional cell growth in Arabidopsis lateral roots
eLife, Jul 29, 2019; DOI: 10.7554/eLife.47988
Quantification of phenotypes
Author: Zhe Chen
Date: Jan 7, 2020，Views：1025
Original protocol used in research article：
Temporal regulation of axonal repulsion by alternative splicing of a conserved microexon in mammalian Robo1 and Robo2
eLife, Aug 8, 2019; DOI: 10.7554/eLife.46042
Soft agar assay
Authors: Shi Yun Yeo, Yoko Itahana and Koji Itahana
Date: Jan 7, 2020，Views：3314
Original protocol used in research article：
Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation
eLife, Mar 9, 2016; DOI: 10.7554/eLife.07101
Custom software
Author: Gordon Wang
Date: Jan 6, 2020，Views：1160
Original protocol used in research article：
Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses
eLife, Oct 22, 2016; DOI: 10.7554/eLife.20560
Single molecule RNA fluorescent in situ hybridization (FISH) and imaging
Author: Michele L Lemons
Date: Jan 6, 2020，Views：1111
Original protocol used in research article：
Neurexin directs partner-specific synaptic connectivity in C. elegans
eLife, Jul 24, 2018; DOI: 10.7554/eLife.35692
Stability calculations
Author: Amelie Stein
Date: Dec 30, 2019，Views：1169
Original protocol used in research article：
Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome
eLife, Nov 7, 2019; DOI: 10.7554/eLife.49138
FRET measurement
Authors: Wanli Liu, Wei Chen and Jizhong Lou
Date: Dec 29, 2019，Views：1281
Original protocol used in research article：
FcγRIIB-I232T polymorphic change allosterically suppresses ligand binding
eLife, Jul 25, 2019; DOI: 10.7554/eLife.46689
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