Protocols shared by authors via RaP service
Journal
Laser speckle perfusion measurements of brain
Author: Stefan Offermanns
Date: Dec 29, 2019，Views：2322
Original protocol used in research article：
Myogenic vasoconstriction requires G12/G13 and LARG to maintain local and systemic vascular resistance
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49374
5’/3’ RACE
Author: Judith Wexler
Date: Dec 24, 2019，Views：1367
Original protocol used in research article：
Hemimetabolous insects elucidate the origin of sexual development via alternative splicing
eLife, Sep 3, 2019; DOI: 10.7554/eLife.47490
β-galactosidase detection in embryos and tissues using X-Gal
Author: Shahragim Tajbakhsh
Date: Dec 22, 2019，Views：1019
Original protocol used in research article：
A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460
Electrophoretic mobility shift assay
Author: Yan Zhang
Date: Dec 19, 2019，Views：1141
Original protocol used in research article：
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
eLife, Aug 6, 2019; DOI: 10.7554/eLife.48725
Single cell isolation and flow cytometry for scRNA-Seq from utricular sensory epithelia
Authors: Mirko Scheibinger, Stefan Heller and Peter G Barr-Gillespie
Date: Dec 17, 2019，Views：1176
Original protocol used in research article：
Single-cell proteomics reveals changes in expression during hair-cell development
eLife, Nov 4, 2019; DOI: 10.7554/eLife.50777
Intact protein mass spectrometry
Author: Michael A Marletta
Date: Dec 17, 2019，Views：1780
Original protocol used in research article：
Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy
eLife, Sep 30, 2019; DOI: 10.7554/eLife.50634
Natural transformation assay
Author: Melanie Blokesch
Date: Dec 17, 2019，Views：1256
Original protocol used in research article：
Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae
eLife, Sep 3, 2019; DOI: 10.7554/eLife.48212
Fluorometry of solubilized cell extracts
Author: Seksiri Arttamangkul
Date: Dec 16, 2019，Views：1026
Original protocol used in research article：
Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
eLife, Oct 7, 2019; DOI: 10.7554/eLife.49319
Live-cell imaging
Author: Elçin Ünal
Date: Dec 16, 2019，Views：1124
Original protocol used in research article：
Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast
eLife, Aug 9, 2019; DOI: 10.7554/eLife.47156
Quantification of neural crest contributions to the ventricular myocardium and regeneration
Author: Marianne E Bronner
Date: Dec 14, 2019，Views：1326
Original protocol used in research article：
Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
eLife, Aug 8, 2019; DOI: 10.7554/eLife.47929
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.