Protocols shared by authors via RaP service
Journal
mRNA preparation
Author: Maaike Welling
Date: Feb 20, 2020，Views：3593
Original protocol used in research article：
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
eLife, Jan 21, 2019; DOI: 10.7554/eLife.44491
In vitro kinase assay using immunoprecipitated Cdk5 from SCN
Authors: Andrea Brenna and Urs Albrecht
Date: Feb 20, 2020，Views：1247
Original protocol used in research article：
Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock
eLife, Nov 5, 2019; DOI: 10.7554/eLife.50925
Live imaging of tracheal invasion into pupal IFMs
Authors: Stefan Luschnig and Julia Sauerwald
Date: Feb 19, 2020，Views：1212
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Immunofluorescence and imaging
Author: Aimin Peng
Date: Feb 18, 2020，Views：1108
Original protocol used in research article：
Kinesin Kif2C in regulation of DNA double strand break dynamics and repair
eLife, Jan 17, 2020; DOI: 10.7554/eLife.53402
Tissue processing for small capillary immunochemistry
Authors: Guillermo Luxán, Mara E Pitulescu and Ralf H Adams
Date: Feb 18, 2020，Views：1224
Original protocol used in research article：
Endothelial EphB4 maintains vascular integrity and transport function in adult heart
eLife, Nov 29, 2019; DOI: 10.7554/eLife.45863
Trypsin digestion
Author: Giulia Bertolin
Date: Feb 17, 2020，Views：1661
Original protocol used in research article：
Aurora kinase A localises to mitochondria to control organelle dynamics and energy production
eLife, Aug 2, 2018; DOI: 10.7554/eLife.38111
Cell culture
Author: Gabriela Dankova
Date: Feb 13, 2020，Views：1274
Original protocol used in research article：
Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898
Radiolabelled nutrient uptake for suspension cells
Author: Linda V Sinclair
Date: Feb 12, 2020，Views：1176
Original protocol used in research article：
Antigen receptor control of methionine metabolism in T cells
eLife, Mar 27, 2019; DOI: 10.7554/eLife.44210
Single-cell transcriptomics platform: 10x Chromium
Authors: Ralph Patrick, Robert E Nordon and Richard P Harvey
Date: Feb 11, 2020，Views：1740
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
Mouse tracking
Authors: Yair Shemesh, Oren Forkosh, Alon Chen and Elad Schneidman
Date: Feb 2, 2020，Views：1689
Original protocol used in research article：
High-order social interactions in groups of mice
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00759
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.