Protocols shared by authors via RaP service
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Single cell isolation from SVF
Author: Prashant Rajbhandari
Date: Feb 25, 2020，Views：1435
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Spike phase-coupling code
Authors: Hugo Balleza-Tapia and André Fisahn
Date: Feb 25, 2020，Views：1189
Original protocol used in research article：
TrpV1 receptor activation rescues neuronal function and network gamma oscillations from Aβ-induced impairment in mouse hippocampus in vitro
eLife, Nov 12, 2018; DOI: 10.7554/eLife.37703
Adipocyte nuclei isolation from iWAT
Author: Prashant Rajbhandari
Date: Feb 24, 2020，Views：2091
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Assembly of stalled yeast Pol II transcription elongation complex
Authors: Zhijie Chen, Ronen Gabizon, Tingting Yao and Carlos Bustamante
Date: Feb 23, 2020，Views：1406
Original protocol used in research article：
High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier
eLife, Jul 31, 2019; DOI: 10.7554/eLife.48281
Subcellular fractionation
Author: Rosa Puertollano
Date: Feb 21, 2020，Views：1324
Original protocol used in research article：
The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage
eLife, Dec 6, 2018; DOI: 10.7554/eLife.40856
Primary NSC Culture
Authors: Isabelle Maria Blomfield and François Guillemot
Date: Feb 21, 2020，Views：1329
Original protocol used in research article：
Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48561
Juxtacellular labelling of single neurons
Author: Navid Farassat
Date: Feb 21, 2020，Views：1194
Original protocol used in research article：
In vivo functional diversity of midbrain dopamine neurons within identified axonal projections
eLife, Oct 3, 2019; DOI: 10.7554/eLife.48408
Chromatin immunoprecipitation (ChIP)
Authors: Xuehua Zhong and Xiangsong Chen
Date: Feb 20, 2020，Views：1332
Original protocol used in research article：
POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging in Arabidopsis
eLife, Nov 22, 2016; DOI: 10.7554/eLife.17214
Automatic determination of optimal cluster number
Author: Jakob Nikolas Kather
Date: Feb 20, 2020，Views：1233
Original protocol used in research article：
Topography of cancer-associated immune cells in human solid tumors
eLife, Sep 4, 2018; DOI: 10.7554/eLife.36967
α-syn purification
Author: Smriti Sangwan
Date: Feb 20, 2020，Views：1176
Original protocol used in research article：
Inhibition of synucleinopathic seeding by rationally designed inhibitors
eLife, Jan 2, 2020; DOI: 10.7554/eLife.46775
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