Protocols shared by authors via RaP service
Journal
NPM1-NeonGreen
Author: C. Zechner
Date: Mar 13, 2020，Views：890
Original protocol used in research article：
Phase separation provides a mechanism to reduce noise in cells
Science, Jan 23, 2020; DOI: 10.1126/science.aav6691
Western blot
Authors: Valerio Azzimato and Myriam Aouadi
Date: Mar 13, 2020，Views：1273
Original protocol used in research article：
Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance
Science Translational Medicine, Feb 25, 2020; DOI: 10.1126/scitranslmed.aaw9709
Isolation and methylation profiling of cfDNA
Authors: Huiyan Luo and Rui-hua Xu
Date: Mar 12, 2020，Views：1092
Original protocol used in research article：
Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533
Protocol for preparing demembranated sperm nuclei
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：961
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
Protocol for making cycling egg extracts from Xenopus laevis
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：1071
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
3,3-Bis(methylsulfonyl)propanamide experimental
Author: Clifton E. Barry
Date: Mar 11, 2020，Views：1030
Original protocol used in research article：
PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis
Science, Mar 5, 2020; DOI: 10.1126/science.aav5912
Lentiviral transduction
Authors: Saravanan Gunaseelan, Jie Yin Chua and John Jia En Chua
Date: Mar 9, 2020，Views：2831
Original protocol used in research article：
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity
eLife, Aug 27, 2019; DOI: 10.7554/eLife.47498
Image analyses
Author: Sari Tojkander
Date: Mar 9, 2020，Views：1096
Original protocol used in research article：
Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly
eLife, Dec 10, 2015; DOI: 10.7554/eLife.06126
Collagen gel chamber assembly
Author: Enas Abu-Shah
Date: Mar 6, 2020，Views：1060
Original protocol used in research article：
A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48221
Tissue contrasting for µ-CT scanning
Author: Marketa Kaucka
Date: Mar 6, 2020，Views：1090
Original protocol used in research article：
Signals from the brain and olfactory epithelium control shaping of the mammalian nasal capsule cartilage
eLife, Jun 13, 2018; DOI: 10.7554/eLife.34465
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