Protocols shared by authors via RaP service
Journal
Skeletal stain followed by micro-computed tomography
Authors: Camilla S Teng, Robert E Maxson and J Gage Crump
Date: Oct 18, 2020，Views：930
Original protocol used in research article：
Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome
eLife, Oct 25, 2018; DOI: 10.7554/eLife.37024
Western blots
Author: Weilin Zhang
Date: Oct 15, 2020，Views：985
Original protocol used in research article：
Hypoxic mitophagy regulates mitochondrial quality and platelet activation and determines severity of I/R heart injury
eLife, Dec 20, 2016; DOI: 10.7554/eLife.21407
RdRp assay for DENV4 NS5Pol
Authors: Cheng-Feng Qin and Zhong-Yu Liu
Date: Oct 15, 2020，Views：976
Original protocol used in research article：
Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization
eLife, Oct 1, 2016; DOI: 10.7554/eLife.17636
Bioinformatics analyses
Authors: Karin Hansson and Daniel Bexell
Date: Oct 15, 2020，Views：791
Original protocol used in research article：
Therapeutic targeting of KSP in preclinical models of high-risk neuroblastoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aba4434
Retrograde labeling of motor neurons in adult zebrafish using dextran or DiI
Authors: Buntaro Zempo and Fumihito Ono
Date: Oct 14, 2020，Views：896
Original protocol used in research article：
Synaptic silencing of fast muscle is compensated by rewired innervation of slow muscle
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aax8382
Protocol of full article
Author: Ye Duan
Date: Oct 14, 2020，Views：835
Original protocol used in research article：
Engineering essential genes with a “jump board” strategy using CRISPR/Cas9
microPublication Biology, Oct 8, 2020; DOI: 10.17912/micropub.biology.000315
Nestmate recognition assay
Author: Cassondra L Vernier
Date: Oct 14, 2020，Views：894
Original protocol used in research article：
The cuticular hydrocarbon profiles of honey bee workers develop via a socially-modulated innate process
eLife, Feb 5, 2019; DOI: 10.7554/eLife.41855
Animal studies
Author: Debomita Chakraborty
Date: Oct 13, 2020，Views：778
Original protocol used in research article：
Fibroblast growth factor receptor 3 activates a network of profibrotic signaling pathways to promote fibrosis in systemic sclerosis
Science Translational Medicine, Sep 29, 2020; DOI: 10.1126/scitranslmed.aaz5506
Whole mount immunofluorescence microscopy of mouse E7.5 embryo for cilia stain
Author: Katsutoshi Mizuno
Date: Oct 12, 2020，Views：1148
Original protocol used in research article：
Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.aba1195
Codon optimized sequence of human ORC genes
Author: Matt J Jaremko
Date: Oct 12, 2020，Views：940
Original protocol used in research article：
The dynamic nature of the human origin recognition complex revealed through five cryoEM structures
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58622
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