Protocols shared by authors via RaP service
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Statistical analysis
Authors: Cassondra L. Vernier and Yehuda Ben-Shahar
Date: Oct 30, 2020，Views：837
Original protocol used in research article：
The gut microbiome defines social group membership in honey bee colonies
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3431
Polysome and Puromycin immunoprecipitation
Authors: Benjamin D Hobson and Peter A Sims
Date: Oct 30, 2020，Views：1596
Original protocol used in research article：
Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048
Cell cycle prediction
Authors: Anneke Dixie Kakebeen and Andrea Elizabeth Wills
Date: Oct 29, 2020，Views：1020
Original protocol used in research article：
Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648
3D vascular imaging
Authors: Célian Bimbard, Charlie Demene and Yves Boubenec
Date: Oct 29, 2020，Views：874
Original protocol used in research article：
Multi-scale mapping along the auditory hierarchy using high-resolution functional UltraSound in the awake ferret
eLife, Jun 28, 2018; DOI: 10.7554/eLife.35028
List of antibodies used for cell sorting
Author: Jaromír Mikeš
Date: Oct 29, 2020，Views：840
Original protocol used in research article：
A genome-wide transcriptomic analysis of protein-coding genes in human blood cells
Science, Dec 19, 2019; DOI: 10.1126/science.aax9198
Compound treatment assay for C. elegans
Authors: Di Zhu and Ye Tian
Date: Oct 28, 2020，Views：911
Original protocol used in research article：
NuRD mediates mitochondrial stress–induced longevity via chromatin remodeling in response to acetyl-CoA level
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb2529
In vitro collagenase activity assay
Authors: Honoo Satake and Shin Matsubara
Date: Oct 28, 2020，Views：1353
Original protocol used in research article：
The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates
eLife, Oct 1, 2019; DOI: 10.7554/eLife.49062
Pathogen inoculation procedure
Author: Shouan Liu
Date: Oct 27, 2020，Views：1345
Original protocol used in research article：
Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100
eLife, Jun 15, 2015; DOI: 10.7554/eLife.07295
Purification
Author: Jia-Yin Tsai
Date: Oct 27, 2020，Views：838
Original protocol used in research article：
Structure of the sodium-dependent phosphate transporter reveals insights into human solute carrier SLC20
Science Advances, Aug 6, 2020; DOI: 10.1126/sciadv.abb4024
Imaging of microdroplets and image analysis
Author: SangMoon Lhee
Date: Oct 27, 2020，Views：803
Original protocol used in research article：
Spatial localization of charged molecules by salt ions in oil-confined water microdroplets
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.aba0181
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