Protocols shared by authors via RaP service
Journal
Isoelectric focusing (IEF)
Author: Steffen Preissler
Date: Oct 11, 2020，Views：1013
Original protocol used in research article：
AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
eLife, Dec 17, 2015; DOI: 10.7554/eLife.12621
Cell lysis
Authors: Jonathan M Budzik and Jeffery S Cox
Date: Oct 6, 2020，Views：1163
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
Sample extraction and metabolic profiling
Author: Jan Gruber
Date: Oct 5, 2020，Views：1096
Original protocol used in research article：
Metabolic stress is a primary pathogenic event in transgenic Caenorhabditis elegans expressing pan-neuronal human amyloid beta
eLife, Oct 15, 2019; DOI: 10.7554/eLife.50069
Wound-induced hair neogenesis
Authors: Jenny J. Wei and Thomas H. Leung
Date: Oct 5, 2020，Views：903
Original protocol used in research article：
Activation of TRPA1 nociceptor promotes systemic adult mammalian skin regeneration
Science Immunology, Aug 27, 2020; DOI: 10.1126/sciimmunol.aba5683
Isolation of human hepatocytes by collagenase perfusion
Author: Marti Cabanes-Creus
Date: Oct 5, 2020，Views：1067
Original protocol used in research article：
Restoring the natural tropism of AAV2 vectors for human liver
Science Translational Medicine, Sep 8, 2020; DOI: 10.1126/scitranslmed.aba3312
Drug sensitivity assays
Authors: Ali H Alghamdi and Harry P De Koning
Date: Oct 1, 2020，Views：1000
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Lap shear adhesion measurements
Authors: Liming Bian and Xiayi Xu
Date: Oct 1, 2020，Views：895
Original protocol used in research article：
Bioadhesive hydrogels demonstrating pH-independent and ultrafast gelation promote gastric ulcer healing in pigs
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aba8014
Immunohistochemistry (IHC)
Author: Daniel Hasche
Date: Oct 1, 2020，Views：1684
Original protocol used in research article：
Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity
eLife, Aug 4, 2020; DOI: 10.7554/eLife.57626
Ovine model of osteochondral defect repair
Author: Frances Henson
Date: Sep 30, 2020，Views：817
Original protocol used in research article：
Agrin induces long-term osteochondral regeneration by supporting repair morphogenesis
Science Translational Medicine, Sep 1, 2020; DOI: 10.1126/scitranslmed.aax9086
Cryo-EM sample preparation
Authors: Byung-Gil Lee and Jan Löwe
Date: Sep 30, 2020，Views：1172
Original protocol used in research article：
Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3
eLife, Sep 15, 2020; DOI: 10.7554/eLife.59560
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