Protocols shared by authors via RaP service
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Measurement of root growth and cell death area in the root tip using Arabidopsis seedlings that grow under DNA stress
Authors: Naoki Takahashi and Masaaki Umeda
Date: Oct 23, 2020，Views：1068
Original protocol used in research article：
A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis
eLife, Apr 4, 2019; DOI: 10.7554/eLife.43944
Fluorescamine-based assay
Authors: Yiwei Yang, Falin Tian, Di Nie, Yuan Liu, Kun Qian, Miaorong Yu, Aohua Wang, Yaqi Zhang, Xinghua Shi and Yong Gan
Date: Oct 22, 2020，Views：840
Original protocol used in research article：
Rapid transport of germ-mimetic nanoparticles with dual conformational polyethylene glycol chains in biological tissues
Science Advances, Feb 6, 2020; DOI: 10.1126/sciadv.aay9937
Dose response curve fitting
Author: Ramnik J Xavier
Date: Oct 21, 2020，Views：1033
Original protocol used in research article：
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells
eLife, May 22, 2015; DOI: 10.7554/eLife.05920
Cloning and purification of VapB22, VapC22, and VapBC22
Authors: Krishan Gopal Thakur and Ramandeep Singh
Date: Oct 21, 2020，Views：864
Original protocol used in research article：
VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response
Science Advances, Jun 2, 2020; DOI: 10.1126/sciadv.aba6944
Electrophysiology
Authors: Shangbang Gao and Zhitao Hu
Date: Oct 21, 2020，Views：868
Original protocol used in research article：
Minimum number of synaptic vesicles for the initiation of a single action potential at C. elegans neuromuscular junction
microPublication Biology, Oct 6, 2020; DOI: 10.17912/micropub.biology.000316
Cell preparation for analysis
Author: Alok Kumar
Date: Oct 20, 2020，Views：950
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Quantification of the diversities of V(D)J recombination events for a given CDR3 peptide
Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：1004
Original protocol used in research article：
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083
CDR3 tree map analysis
Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：964
Original protocol used in research article：
Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083
Cuticular hydrocarbon extractions and GC analysis
Authors: Cassondra L Vernier, Yehuda Ben-Shahar and Joshua Krupp
Date: Oct 20, 2020，Views：999
Original protocol used in research article：
The cuticular hydrocarbon profiles of honey bee workers develop via a socially-modulated innate process
eLife, Feb 5, 2019; DOI: 10.7554/eLife.41855
Zymography
Author: Trayambak Pathak
Date: Oct 20, 2020，Views：1072
Original protocol used in research article：
Dichotomous role of the human mitochondrial Na+/Ca2+/Li+ exchanger NCLX in colorectal cancer growth and metastasis
eLife, Sep 11, 2020; DOI: 10.7554/eLife.59686
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