Protocols shared by authors via RaP service
Journal
Immunofluorescence
Authors: Ruihua Ma and Beatriz Sosa-Pineda
Date: Sep 17, 2020，Views：1064
Original protocol used in research article：
Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
eLife, Mar 10, 2020; DOI: 10.7554/eLife.46206
RNAscope in situ hybridization
Author: Glenda Comai
Date: Sep 16, 2020，Views：1852
Original protocol used in research article：
A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460
Flow cytometry antibody panel
Authors: Alexandre Boissonnas and Pierre Louis Loyher
Date: Sep 16, 2020，Views：981
Original protocol used in research article：
Immune surveillance of the lung by migrating tissue monocytes
eLife, Jul 13, 2015; DOI: 10.7554/eLife.07847
Cryo–electron tomography
Authors: M. A. Benhaim, K. K. Lee and Vidya Mangala Prasad
Date: Sep 15, 2020，Views：835
Original protocol used in research article：
Structural monitoring of a transient intermediate in the hemagglutinin fusion machinery on influenza virions
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8822
Exosome isolation, Subcellular fractionation, and TCA precipitation
Author: Karoliina Stefanius
Date: Sep 14, 2020，Views：1148
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
Quantification of NaV1.1 Protein in Mouse Brains using Meso Scale Discovery (MSD) Method
Authors: Zhou Han and Gene Liau
Date: Sep 14, 2020，Views：1046
Original protocol used in research article：
Antisense oligonucleotides increase Scn1a expression and reduce seizures and SUDEP incidence in a mouse model of Dravet syndrome
Science Translational Medicine, Aug 26, 2020; DOI: 10.1126/scitranslmed.aaz6100
NK stimulation assay
Author: Sytse Jan Piersma
Date: Sep 14, 2020，Views：942
Original protocol used in research article：
A herpesvirus encoded Qa-1 mimic inhibits natural killer cell cytotoxicity through CD94/NKG2A receptor engagement
eLife, Dec 21, 2018; DOI: 10.7554/eLife.38667
Expression and Purification of the Human Cation-Chloride Cotransporter KCC1 from HEK293F cells for Structural Studies
Authors: Si Liu and Jiangtao Guo
Date: Sep 14, 2020，Views：1209
Original protocol used in research article：
Cryo-EM structures of the human cation-chloride cotransporter KCC1
Science, Oct 25, 2019; DOI: 10.1126/science.aay3129
Human monocyte-derived dendritic cell (moDC) differentiation and suppression of moDCs by Tregs
Authors: Qing Jessica Huang and Megan K. Levings
Date: Sep 13, 2020，Views：1083
Original protocol used in research article：
Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells
Science Translational Medicine, Aug 19, 2020; DOI: 10.1126/scitranslmed.aaz3866
IgH recombination
Author: Masaki Miyazaki
Date: Sep 13, 2020，Views：892
Original protocol used in research article：
The transcription factor E2A activates multiple enhancers that drive Rag expression in developing T and B cells
Science Immunology, Sep 3, 2020; DOI: 10.1126/sciimmunol.abb1455
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