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Detection of proteins in membrane fractions
Authors: Alam García-Heredia, Takehiro Kado, Caralyn E. Sein, Julia Puffal, Sarah H. Osman, Julius Judd, Todd A. Gray, Yasu S. Morita and M. Sloan Siegrist
Date: Feb 23, 2021，Views：954
Original protocol used in research article：
Membrane-partitioned cell wall synthesis in mycobacteria
eLife, Feb 5, 2021; DOI: 10.7554/eLife.60263
Protocol of full article
Author: Kenneth Trimmer
Date: Feb 23, 2021，Views：798
Original protocol used in research article：
CRISPR-edit point mutant allele detection (CEPAD)-PCR method for rapid screening of CRISPR edited point mutations
microPublication Biology, Jan 1, 1970; DOI: 10.17912/micropub.biology.000368
Subcellular fractionation
Authors: Tyler B Faust and Alan D Frankel
Date: Feb 22, 2021，Views：883
Original protocol used in research article：
The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI: 10.7554/eLife.31879
Sequences, plasmids, transfection and RNA interference
Author: Zhou Yu
Date: Feb 22, 2021，Views：859
Original protocol used in research article：
Lys29-linkage of ASK1 by Skp1−Cullin 1−Fbxo21 ubiquitin ligase complex is required for antiviral innate response
eLife, Apr 11, 2016; DOI: 10.7554/eLife.14087
Immune signature, immune infiltration, and nontumor fraction
Authors: Yue Wang and Da Yang
Date: Feb 22, 2021，Views：858
Original protocol used in research article：
LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abb3555
Bisulfite sequencing and TAB sequencing
Authors: Gerd P. Pfeifer and Zhijun Huang
Date: Feb 22, 2021，Views：864
Original protocol used in research article：
The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins
Science Advances, Jan 20, 2021; DOI: 10.1126/sciadv.abb9149
Synthesis of FX and preparation of FX@HP
Authors: Minjie Sun and Zhaoting Li
Date: Feb 22, 2021，Views：875
Original protocol used in research article：
Targeting pulmonary tumor microenvironment with CXCR4-inhibiting nanocomplex to enhance anti–PD-L1 immunotherapy
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aaz9240
Procurement and treatment of human cartilage
Author: Fadia Kamal
Date: Feb 22, 2021，Views：863
Original protocol used in research article：
Paroxetine-mediated GRK2 inhibition is a disease-modifying treatment for osteoarthritis
Science Translational Medicine, Feb 9, 2021; DOI: 10.1126/scitranslmed.aau8491
Animal studies
Author: Fadia Kamal
Date: Feb 22, 2021，Views：889
Original protocol used in research article：
Paroxetine-mediated GRK2 inhibition is a disease-modifying treatment for osteoarthritis
Science Translational Medicine, Feb 9, 2021; DOI: 10.1126/scitranslmed.aau8491
Preparation of lymph node stromal cells
Authors: Christian Perez-Shibayama and Burkhard Ludewig
Date: Feb 22, 2021，Views：808
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
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