Protocols shared by authors via RaP service
Journal
Isolation of thymic epithelial cells and thymocytes
Authors: Chris P Ponting and Georg A Holländer
Date: Feb 26, 2021，Views：1780
Original protocol used in research article：
Ageing compromises mouse thymus function and remodels epithelial cell differentiation
eLife, Aug 25, 2020; DOI: 10.7554/eLife.56221
Gene construction
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：958
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Antibody sequences
Authors: Yan Wu, Feng Gao and George F. Gao
Date: Feb 25, 2021，Views：1001
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Cells and Viruses
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1104
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Protein expression and purification
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1571
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Particle tracking
Author: Masazumi Fujiwara
Date: Feb 25, 2021，Views：856
Original protocol used in research article：
Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI: 10.1126/sciadv.aba9636
ZFA enrichment
Author: Richard J White
Date: Feb 25, 2021，Views：1061
Original protocol used in research article：
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
Plasmid constructs
Author: Theodore S. Jardetzky
Date: Feb 24, 2021，Views：907
Original protocol used in research article：
EphrinB2 clustering by Nipah virus G is required to activate and trap F intermediates at supported lipid bilayer–cell interfaces
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abe1235
List of antibodies, siRNAs and shRNAs
Author: Seyed Mehdi Jafarnejad
Date: Feb 24, 2021，Views：902
Original protocol used in research article：
Translational control of ERK signaling through miRNA/4EHP-directed silencing
eLife, Feb 7, 2018; DOI: 10.7554/eLife.35034
RNA extraction from individual zebrafish embryos
Authors: Neha Wali, Richard White and Elisabeth M Busch-Nentwich
Date: Feb 24, 2021，Views：1267
Original protocol used in research article：
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
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