Protocols shared by authors via RaP service
Journal
Cell lysis, IP, immunoblotting, and antibodies
Author: Yan-ping Xu
Date: Mar 3, 2021，Views：876
Original protocol used in research article：
USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2
Science Advances, Sep 17, 2020; DOI: 10.1126/sciadv.abc9730
Human Cytomegalovirus (HCMV) infection of CD14+ monocytes and reactivation
Authors: Miri Shnayder, Noam Stern-Ginossar and Michal Schwartz
Date: Mar 3, 2021，Views：899
Original protocol used in research article：
Single cell analysis reveals human cytomegalovirus drives latently infected cells towards an anergic-like monocyte state
eLife, Jan 22, 2020; DOI: 10.7554/eLife.52168
Preparation of BS biofilms
Author: Jinyao Liu
Date: Mar 3, 2021，Views：902
Original protocol used in research article：
Bioinspired oral delivery of gut microbiota by self-coating with biofilms
Science Advances, Jun 23, 2020; DOI: 10.1126/sciadv.abb1952
Data repositories
Author: Andrew N Holding
Date: Mar 3, 2021，Views：839
Original protocol used in research article：
Genome-wide Estrogen Receptor-α activation is sustained, not cyclical
eLife, Nov 20, 2018; DOI: 10.7554/eLife.40854
Design and fabrication of the electronic modules
Authors: Wei Lu, Wubin Bai, Zhi-Dong Ge and John A. Rogers
Date: Mar 2, 2021，Views：880
Original protocol used in research article：
Wireless implantable catheter-type oximeter designed for cardiac oxygen saturation
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abe0579
Fabrication of catheter-type oximeter probe
Authors: Wei Lu, Wubin Bai, Zhi-Dong Ge and John A. Rogers
Date: Mar 2, 2021，Views：914
Original protocol used in research article：
Wireless implantable catheter-type oximeter designed for cardiac oxygen saturation
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abe0579
Assembly, transformation, and selection of the IL-6 library
Authors: Jonathan Martinez-Fabregas, Elizabeth Pohler and Ignacio Moraga
Date: Mar 2, 2021，Views：904
Original protocol used in research article：
Kinetics of cytokine receptor trafficking determine signaling and functional selectivity
eLife, Nov 27, 2019; DOI: 10.7554/eLife.49314
Affinity purification and mass spectrometry analysis
Author: Mathieu Rougemaille
Date: Mar 2, 2021，Views：849
Original protocol used in research article：
Ubiquitination-dependent control of sexual differentiation in fission yeast
eLife, Aug 25, 2017; DOI: 10.7554/eLife.28046
Preparation of XaxAB pore complexes
Author: Evelyn Schubert
Date: Mar 2, 2021，Views：924
Original protocol used in research article：
Membrane insertion of α-xenorhabdolysin in near-atomic detail
eLife, Jul 16, 2018; DOI: 10.7554/eLife.38017
Read data processing
Author: Maximilian R Stammnitz
Date: Mar 2, 2021，Views：1007
Original protocol used in research article：
Freshwater monitoring by nanopore sequencing
eLife, Jan 19, 2021; DOI: 10.7554/eLife.61504
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