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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1376)
Biophysics (164)
Cancer Biology (431)
Cell Biology (2122)
Developmental Biology (321)
Immunology (597)
Microbiology (1154)
Molecular Biology (1132)
Neuroscience (703)
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RNA-seq and read mapping
Authors:
Mao-Bin Xie
and
Ming Liu
Date: Nov 2, 2022,Views:416
Original protocol used in research article:
Targeting tumor lineage plasticity in hepatocellular carcinoma using an anti-CLDN6 antibody-drug conjugate
Science Translational Medicine, Feb 3, 2021; DOI:
10.1126/scitranslmed.abb6282
GFP-VLPs and flow cytometry
Authors:
Abdullah M. Syed
,
Melanie Ott
and
Jennifer A. Doudna
Date: Nov 2, 2022,Views:501
Original protocol used in research article:
Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles
Science, Nov 4, 2021; DOI:
10.1126/science.abl6184
Fluorescence microscopy
Author:
Benjamin S Glick
Date: Nov 2, 2022,Views:372
Original protocol used in research article:
COPI selectively drives maturation of the early Golgi
eLife, Dec 28, 2015; DOI:
10.7554/eLife.13232
Immunofluorescence and whole mount aorta imaging
Authors:
Marco Orecchioni
and
Klaus Ley
Date: Nov 2, 2022,Views:361
Original protocol used in research article:
Olfactory receptor 2 in vascular macrophages drives atherosclerosis by NLRP3-dependent IL-1 production
Science, Jan 13, 2022; DOI:
10.1126/science.abg3067
Human cortical cell culture
Authors:
Ryohei Iwata
and
Pierre Verhaeghen
Date: Nov 2, 2022,Views:427
Original protocol used in research article:
Mitochondrial dynamics in postmitotic cells regulate neurogenesis
Science, Aug 13, 2020; DOI:
10.1126/science.aba9760
MSC/Macrophage co-cultures
Authors:
S. Jyothi Prasanna
,
Anoop Vasandan
and
Shashank Chandanala
Date: Nov 1, 2022,Views:409
Original protocol used in research article:
Human Mesenchymal stem cells program macrophage plasticity by altering their metabolic status via a PGE2-dependent mechanism
Scientific Reports, Dec 2, 2016; DOI:
10.1038/srep38308
Calibration targets and methods
Authors:
Rebecca C. Harris
and
Richard G. White
Date: Oct 30, 2022,Views:373
Original protocol used in research article:
Potential impact of tuberculosis vaccines in China South Africa and India
Science Translational Medicine, Oct 6, 2020; DOI:
10.1126/scitranslmed.aax4607
Morphological Analysis
Authors:
Haibin Chen
and
Yanan Wang
Date: Oct 29, 2022,Views:344
Original protocol used in research article:
The Role of Syncytin in Placental Angiogenesis and Fetal Growth
Frontiers in Cell and Developmental Biology, Apr 12, 2022; DOI:
10.3389/fcell.2022.852561
Antibody labeling and binding assay with b1b2 domain of NRP-1
Authors:
Ludovico Cantuti-Castelvetri
,
Tambet Teesalu
,
Giuseppe Balistreri
and
Mikael Simons
Date: Oct 29, 2022,Views:345
Original protocol used in research article:
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI:
10.1126/science.abd2985
Mouse immunohistochemistry and immunofluorescence
Authors:
Ludovico Cantuti-Castelvetri
,
Tambet Teesalu
,
Giuseppe Balistreri
and
Mikael Simons
Date: Oct 29, 2022,Views:338
Original protocol used in research article:
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI:
10.1126/science.abd2985
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