Protocols shared by authors via RaP service
Journal
Isolation of renal-infiltrating T cells
Authors: Ping-Min Chen and Joe Craft
Date: Nov 15, 2022，Views：857
Original protocol used in research article：
Kidney tissue hypoxia dictates T cell–mediated injury in murine lupus nephritis
Science Translational Medicine, Apr 7, 2020; DOI: 10.1126/scitranslmed.aay1620
Cell culture and reagents
Authors: Yool Lee and Amita Sehgal
Date: Nov 15, 2022，Views：488
Original protocol used in research article：
Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abd2645
Real-time reverse transcription PCR
Author: Xintao Shuai
Date: Nov 14, 2022，Views：667
Original protocol used in research article：
Dual pH-sensitive nanodrug blocks PD-1 immune checkpoint and uses T cells to deliver NF-κB inhibitor for antitumor immunotherapy
Science Advances, Feb 4, 2020; DOI: 10.1126/sciadv.aay7785
Code availability
Authors: DongAhn Yoo, Jeremy S Dasen, Heebal Kim and Myungin Baek
Date: Nov 8, 2022，Views：470
Original protocol used in research article：
Little skate genome provides insights into genetic programs essential for limb-based locomotion
eLife, Oct 26, 2022; DOI: 10.7554/eLife.78345
Detection of phosphoepitopes by Flow cytometry
Authors: Antoine Marçais and Thierry Walzer
Date: Nov 7, 2022，Views：607
Original protocol used in research article：
High mTOR activity is a hallmark of reactive natural killer cells and amplifies early signaling through activating receptors
eLife, Sep 6, 2017; DOI: 10.7554/eLife.26423
ENCODE dynamic k-mer–assisted mapping
Authors: Ariel Gershman, Michael E. G. Sauria, Karen H. Miga and Winston Timp
Date: Nov 7, 2022，Views：493
Original protocol used in research article：
Epigenetic patterns in a complete human genome
Science, Apr 1, 2022; DOI: 10.1126/science.abj5089
Repeated stimulation assay
Authors: Giedre Krenciute, Stephen Gottschalk and Ben Youngblood
Date: Nov 6, 2022，Views：654
Original protocol used in research article：
Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity
Science Translational Medicine, Nov 17, 2021; DOI: 10.1126/scitranslmed.abh0272
pMHCII-CAR constructs
Authors: Jaeu Yi, Gregory F. Wu and Chyi-Song Hsieh
Date: Nov 5, 2022，Views：578
Original protocol used in research article：
Antigen-specific depletion of CD4+ T cells by CAR T cells reveals distinct roles of higher- and lower-affinity TCRs during autoimmunity
Science Immunology, Oct 7, 2022; DOI: 10.1126/sciimmunol.abo0777
Microtubule co-sedimentation assay
Authors: Shaohe Wang and Karen Oegema
Date: Nov 4, 2022，Views：724
Original protocol used in research article：
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Protein expression and purification of OCT4 and SOX2
Authors: Dirk Schübeler and Nicolas H. Thomä
Date: Nov 3, 2022，Views：560
Original protocol used in research article：
Mechanisms of OCT4-SOX2 motif readout on nucleosomes
Science, Jun 25, 2020; DOI: 10.1126/science.abb0074
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