Protocols shared by authors via RaP service
Journal
Wire hang test
Authors: Michael A Petr and Rafael de Cabo
Date: Dec 18, 2022，Views：1371
Original protocol used in research article：
A cross-sectional study of functional and metabolic changes during aging through the lifespan in male mice
eLife, Apr 20, 2021; DOI: 10.7554/eLife.62952
HMT assay
Authors: Danny Reinberg|| and Chul-Hwan Lee
Date: Dec 16, 2022，Views：775
Original protocol used in research article：
Multiple modes of PRC2 inhibition elicit global chromatin alterations in H3K27M pediatric glioma
Science Advances, Oct 31, 2018; DOI: 10.1126/sciadv.aau5935
In-vitro stimulation of epithelial cells
Authors: Shahanshah Khan and Hasan Zaki
Date: Dec 16, 2022，Views：796
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Plasmid nucleofection
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1013
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
Lipofectamine transfection of organoids
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1400
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
The Intolerance of Uncertainty Scale – Short Form (IUS-12)
Authors: Einav Gozansky and Hadas Okon-Singer
Date: Dec 14, 2022，Views：1209
Original protocol used in research article：
Identifying Variables That Predict Depression Following the General Lockdown During the COVID-19 Pandemic
Frontiers in Psychology, May 17, 2021; DOI: 10.3389/fpsyg.2021.680768
Prediction of Mtb-secreted eukaryotic-like proteins
Authors: Qiyao Chai, Zhe Lu, Jing Wang and Cui Hua Liu
Date: Dec 14, 2022，Views：821
Original protocol used in research article：
A bacterial phospholipid phosphatase inhibits host pyroptosis by hijacking ubiquitin
Science, Oct 14, 2022; DOI: 10.1126/science.abq0132
Tissue processing and histological analysis
Author: Jian Yu
Date: Dec 13, 2022，Views：872
Original protocol used in research article：
Interferon b drives intestinal regeneration after radiation
Science Advances, Oct 6, 2021; DOI: 10.1126/sciadv.abi5253
Fibrin clot formation and external fibrinolysis
Authors: Helen R McPherson and Robert AS Ariëns
Date: Dec 13, 2022，Views：857
Original protocol used in research article：
Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis
eLife, Oct 11, 2021; DOI: 10.7554/eLife.68761
Flow cytometric analysis to detect KIR+CD8+ T cells and KIR subtypes in human PBMCs
Authors: Jing Li, Naresha Saligrama and Mark M. Davis
Date: Dec 12, 2022，Views：957
Original protocol used in research article：
KIR+CD8+ T cells suppress pathogenic T cells and are active in autoimmune diseases and COVID-19
Science, Mar 8, 2022; DOI: 10.1126/science.abi9591
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