Protocols shared by authors via RaP service
Journal
Macrophage scRNA-seq by 10× Genomics
Authors: Chang-Yi Cui and Myriam Gorospe
Date: Jan 7, 2023，Views：665
Original protocol used in research article：
Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations
eLife, Oct 19, 2022; DOI: 10.7554/eLife.77974
Agarose embedding
Authors: Robin J Vigouroux, Alain Chédotal and Kim Tuyen Nguyen-Ba-Charvet
Date: Jan 7, 2023，Views：706
Original protocol used in research article：
Revisiting the role of Dcc in visual system development with a novel eye clearing method
eLife, Feb 25, 2020; DOI: 10.7554/eLife.51275
Stereotactic injections of AAV in hippocampal CA1
Authors: Fujun Luo and Alessandra Sclip
Date: Jan 6, 2023，Views：653
Original protocol used in research article：
Neurexins regulate presynaptic GABAB-receptors at central synapses
Nature Communications, Apr 22, 2021; DOI: 10.1038/s41467-021-22753-5
Gastrointestinal Symptom Rating Scale-IBS
Author: Melissa Hunt
Date: Jan 4, 2023，Views：1159
Original protocol used in research article：
Efficacy of Zemedy, a Mobile Digital Therapeutic for the Self-management of Irritable Bowel Syndrome: Crossover Randomized Controlled Trial
JMIR mHealth and uHealth, May 20, 2021; DOI: 10.2196/26152
siRNA transfection in Calu-3 cells
Authors: Laura Martin-Sancho and Sumit K. Chanda
Date: Jan 4, 2023，Views：812
Original protocol used in research article：
Functional landscape of SARS-CoV-2 cellular restriction
Mol Cell, Apr 13, 2021; DOI: 10.1016/j.molcel.2021.04.008
Immunofluorescence of Drosophila tissues
Author: Julia B Cordero
Date: Jan 4, 2023，Views：680
Original protocol used in research article：
RAL GTPases mediate EGFR-driven intestinal stem cell proliferation and tumourigenesis
eLife, Jun 7, 2021; DOI: 10.7554/eLife.63807
Histological assessment of adipocyte size, non‐alcoholic fatty liver disease and villi structure
Authors: Natalie J Haywood and Mark T Kearney
Date: Jan 3, 2023，Views：682
Original protocol used in research article：
Endothelial IGF‐1 receptor mediates crosstalk with the gut wall to regulate microbiota in obesity
EMBO Reports, May 2, 2021; DOI: 10.15252/embr.202050767
Clinical Study design
Author: Heidi H. Kong
Date: Jan 3, 2023，Views：594
Original protocol used in research article：
Alterations of human skin microbiome and expansion of antimicrobial resistance after systemic antibiotics
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abd8077
Apoptosis induction
Authors: Britt A Glaunsinger and Christopher Duncan-Lewis
Date: Jan 2, 2023，Views：613
Original protocol used in research article：
Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis
eLife, Jun 4, 2021; DOI: 10.7554/eLife.58342
Lentivirus packaging
Author: Jakub Gemperle
Date: Jan 2, 2023，Views：696
Original protocol used in research article：
On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
eLife, ; DOI: 10.7554/eLife.76651
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.