Protocols shared by authors via RaP service
Journal
Clinical Study design
Author: Heidi H. Kong
Date: Jan 3, 2023，Views：585
Original protocol used in research article：
Alterations of human skin microbiome and expansion of antimicrobial resistance after systemic antibiotics
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abd8077
Apoptosis induction
Authors: Britt A Glaunsinger and Christopher Duncan-Lewis
Date: Jan 2, 2023，Views：598
Original protocol used in research article：
Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis
eLife, Jun 4, 2021; DOI: 10.7554/eLife.58342
Lentivirus packaging
Author: Jakub Gemperle
Date: Jan 2, 2023，Views：637
Original protocol used in research article：
On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
eLife, ; DOI: 10.7554/eLife.76651
20-hydroxyecdysone (20E) feeding of Drosophila larvae
Authors: Jacob S Jaszczak, Laura DeVault, Lily Jan and Yuh Nung Jan
Date: Dec 28, 2022，Views：697
Original protocol used in research article：
Steroid hormone signaling activates thermal nociception during Drosophila peripheral nervous system development
eLife, Mar 30, 2022; DOI: 10.7554/eLife.76464
Monocyte isolation, macrophage differentiation, and OC generation
Authors: Qingqing Wang, Shunwu Fan, Xin Liu and Xianfeng Lin
Date: Dec 28, 2022，Views：705
Original protocol used in research article：
Suppression of osteoclast multinucleation via a posttranscriptional regulation–based spatiotemporally selective delivery system
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn3333
Overview of the prediction pipeline
Authors: Diego del Alamo, Davide Sala, Hassane S Mchaourab and Jens Meiler
Date: Dec 27, 2022，Views：851
Original protocol used in research article：
Sampling alternative conformational states of transporters and receptors with AlphaFold2
eLife, Mar 3, 2022; DOI: 10.7554/eLife.75751
Model building and refinement
Authors: Qiang Su and Yigong Shi
Date: Dec 25, 2022，Views：704
Original protocol used in research article：
Cryo-EM structure of the human IgM B cell receptor
Science, Aug 18, 2022; DOI: 10.1126/science.abo3923
S-specific immunoglobulin ELISA
Authors: Karen Colwill, Reuben Samson, Queenie Hu, Anne-Claude Gingras, Natalia Martin Orozco, Jun Liu, Eric G. Marcusson and Mario A. Ostrowski
Date: Dec 23, 2022，Views：711
Original protocol used in research article：
Preclinical evaluation of a SARS-CoV-2 mRNA vaccine PTX-COVID19-B
Science Advances, Jan 19, 2022; DOI: 10.1126/sciadv.abj9815
par-seqFISH
Authors: Daniel Dar, Long Cai and Dianne K. Newman
Date: Dec 20, 2022，Views：765
Original protocol used in research article：
Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution
Science, Aug 12, 2021; DOI: 10.1126/science.abi4882
Real-time quantitative PCR (qRT-PCR)
Authors: Gail Mandel and Tamilla Nechiporuk
Date: Dec 18, 2022，Views：830
Original protocol used in research article：
The REST remodeling complex protects genomic integrity during embryonic neurogenesis
eLife, Jan 8, 2016; DOI: 10.7554/eLife.09584
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