Protocols shared by authors via RaP service
Journal
Site-directed mutagenesis, cell culture and transfection
Author: Da-Wei Zhang
Date: Dec 12, 2022，Views：870
Original protocol used in research article：
Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis
Nature Communications, Mar 25, 2021; DOI: 10.1038/s41467-021-22167-3
IL-1β and IL-18 ELISA
Authors: Kai Sun, Gabriel Mbalaviele and Chun Wang
Date: Dec 12, 2022，Views：985
Original protocol used in research article：
Fracture healing is delayed in the absence of gasdermin-interleukin-1 signaling
eLife, Mar 4, 2022; DOI: 10.7554/eLife.75753
2.6. Bacterial Zeta Potential
Authors: Karina Arellano-Ayala, Juhwan Lim and Wilhelm-Heinrich Holzapfel
Date: Dec 9, 2022，Views：895
Original protocol used in research article：
Rehydration before Application Improves Functional Properties of Lyophilized Lactiplantibacillus plantarum HAC03
Microorganisms, May 8, 2021; DOI: 10.3390/microorganisms9051013
2.3.3. Caregiver’s Feeding Styles Questionnaire (CFSQ)
Authors: Thomas G. Power, Jennifer O. Fisher, Teresia M. O’Connor, Nilda Micheli, Maria A. Papaioannou and Sheryl O. Hughes
Date: Dec 2, 2022，Views：1287
Original protocol used in research article：
General Parenting and Hispanic Mothers’ Feeding Practices and Styles
International Journal of Environmental Research and Public Health, Jan 6, 2021; DOI: 10.3390/ijerph18020380
SC2-VLP concentration using PEG precipitation
Authors: Abdullah M. Syed, Melanie Ott and Jennifer A. Doudna
Date: Dec 1, 2022，Views：1559
Original protocol used in research article：
Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles
Science, Nov 4, 2021; DOI: 10.1126/science.abl6184
Measurement of mitochondrial iron in a murine heart and cultured cardiomyocytes
Authors: Ko Abe, Masataka Ikeda, Tomomi Ide and Hiroyuki Tsutsui
Date: Dec 1, 2022，Views：1034
Original protocol used in research article：
Doxorubicin causes ferroptosis and cardiotoxicity by intercalating into mitochondrial DNA and disrupting Alas1-dependent heme synthesis
Science Signaling, Nov 1, 2022; DOI: 10.1126/scisignal.abn8017
Torsional testing of tibiae
Authors: Dominik Saul, Joshua N Farr and Sundeep Khosla
Date: Dec 1, 2022，Views：930
Original protocol used in research article：
Modulation of fracture healing by the transient accumulation of senescent cells
eLife, Oct 7, 2021; DOI: 10.7554/eLife.69958
Doubling-time assay
Author: Jean Gautier
Date: Dec 1, 2022，Views：984
Original protocol used in research article：
The UVSSA complex alleviates MYC-driven transcription stress
The Journal of Cell Biology, Jan 6, 2021; DOI: 10.1083/jcb.201807163
Peritonitis assay
Authors: Katelyn Arnold, Ding Xu and Jian Liu
Date: Dec 1, 2022，Views：884
Original protocol used in research article：
Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure
Science Translational Medicine, Mar 17, 2020; DOI: 10.1126/scitranslmed.aav8075
Key resource table
Authors: Chenhui Wang and Allan C Spradling
Date: Nov 30, 2022，Views：946
Original protocol used in research article：
An abundant quiescent stem cell population in Drosophila Malpighian tubules protects principal cells from kidney stones
eLife, Mar 16, 2020; DOI: 10.7554/eLife.54096
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