Protocols shared by authors via RaP service
Journal
Hepatic triglyceride content
Authors: Eric Chang, K. Singer and Mita Varghese
Date: Mar 6, 2023，Views：378
Original protocol used in research article：
Programming effects of maternal and gestational obesity on offspring metabolism and metabolic inflammation
Scientific Reports, Nov 5, 2019; DOI: 10.1038/s41598-019-52583-x
DNA fiber assays
Authors: Sharon B. Cantor and Sumeet Nayak
Date: Mar 4, 2023，Views：497
Original protocol used in research article：
Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz7808
Preparation of fibrin gel components
Authors: Ugur Uslu and Carl H. June
Date: Mar 4, 2023，Views：440
Original protocol used in research article：
Chimeric antigen receptor T cells as adjuvant therapy for unresectable adenocarcinoma
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.ade2526
Candidate-gene association mapping
Authors: Xuan Zhang, Jiansheng Li, Jianbing Yan and Xiaohong Yang
Date: Mar 2, 2023，Views：401
Original protocol used in research article：
Convergent selection of a WD40 protein that enhances grain yield in maize and rice
Science, Mar 25, 2022; DOI: 10.1126/science.abg7985
Recombinant protein purification and pulldown
Authors: Gunther Hollopeter, Erik M Jorgensen and Gwendolyn Marie Beacham
Date: Mar 2, 2023，Views：425
Original protocol used in research article：
The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex
eLife, Oct 10, 2014; DOI: 10.7554/eLife.03648
Perfusion and section preparation
Authors: Zheng Gan and Rohini Kuner
Date: Mar 2, 2023，Views：387
Original protocol used in research article：
Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391
Nitro-blue tetrazolium (NBT) assay
Author: Thora Lieke
Date: Mar 1, 2023，Views：598
Original protocol used in research article：
Phenol-rich fulvic acid as a water additive enhances growth, reduces stress, and stimulates the immune system of fish in aquaculture
Scientific Reports, Jan 8, 2021; DOI: 10.1038/s41598-020-80449-0
4.4. The Visual Analogue Scale for Irritable Bowel Syndrome
Author: Bodil Ohlsson
Date: Feb 27, 2023，Views：415
Original protocol used in research article：
AXIN1 in Plasma or Serum Is a Potential New Biomarker for Endometriosis
International Journal of Molecular Sciences, Jan 7, 2019; DOI: 10.3390/ijms20010189
Electrophoretic mobility shift assay
Authors: Tingting Wang, Canfeng Hua, Xin Deng and Jian Yan
Date: Feb 27, 2023，Views：440
Original protocol used in research article：
An atlas of the binding specificities of transcription factors in Pseudomonas aeruginosa directs prediction of novel regulators in virulence
eLife, Mar 29, 2021; DOI: 10.7554/eLife.61885
Collection and procession of peripheral blood mononuclear cell, biliary brush samples, and liver tissue
Author: Niklas K. Björkström
Date: Feb 27, 2023，Views：411
Original protocol used in research article：
A biliary immune landscape map of primary sclerosing cholangitis reveals a dominant network of neutrophils and tissue-resident T cells
Science Translational Medicine, Jun 23, 2021; DOI: 10.1126/scitranslmed.abb3107
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