Protocols shared by authors via RaP service
Journal
2.3. APOE genotyping
Authors: Heather M. Wilkins, Nanda K. Yellapu and Russell H. Swerdlow
Date: Apr 24, 2023，Views：314
Original protocol used in research article：
Bioenergetic and inflammatory systemic phenotypes in Alzheimer’s disease APOE ε4‐carriers
Aging Cell, May 3, 2021; DOI: 10.1111/acel.13356
Ex vivo coculture assay T cells – CD11b cells
Authors: Qiqun Zeng, Agnieszka Chryplewicz and Douglas Hanahan
Date: Apr 24, 2023，Views：310
Original protocol used in research article：
Aberrant hyperexpression of the RNA binding protein FMRP in tumors mediates immune evasion
Science, Nov 18, 2022; DOI: 10.1126/science.abl7207
Flow cytometric analysis
Authors: Shaojie Ding and Xinmei Zhang
Date: Apr 21, 2023，Views：33
Original protocol used in research article：
Macrophage-derived netrin-1 contributes to endometriosis-associated pain
Annals of Translational Medicine, Jan 1, 2021; DOI: 10.21037/atm-20-2161
Primary human islets
Authors: Sonja Schrepfer and Xiaomeng Hu
Date: Apr 21, 2023，Views：365
Original protocol used in research article：
Human hypoimmune primary pancreatic islets avoid rejection and autoimmunity and alleviate diabetes in allogeneic humanized mice
Science Translational Medicine, Apr 12, 2023; DOI: 10.1126/scitranslmed.adg5794
Synthesis of CDP-steroid conjugates
Author: James M. Olson
Date: Apr 21, 2023，Views：40
Original protocol used in research article：
A potent peptide-steroid conjugate accumulates in cartilage and reverses arthritis without evidence of systemic corticosteroid exposure
Science Translational Medicine, Mar 3, 2020; DOI: 10.1126/scitranslmed.aay1041
Three-chamber social approach
Authors: Boris D. Heifets and Robert C. Malenka
Date: Apr 20, 2023，Views：251
Original protocol used in research article：
Distinct neural mechanisms for the prosocial and rewarding properties of MDMA
Science Translational Medicine, Dec 10, 2019; DOI: 10.1126/scitranslmed.aaw6435
Cell line infection with lentiMPRA library, RNA- and DNA-seq, and read processing
Authors: Dmitri A Petrov, Nadav Ahituv and David Gokhman
Date: Apr 20, 2023，Views：53
Original protocol used in research article：
The cis-regulatory effects of modern human-specific variants
eLife, Apr 22, 2021; DOI: 10.7554/eLife.63713
Murine scRNA-seq and data analyses
Authors: Thomas Fabre and Alexander M. S. Barron
Date: Apr 17, 2023，Views：40
Original protocol used in research article：
Identification of a broadly fibrogenic macrophage subset induced by type 3 inflammation
Science Immunology, Apr 7, 2023; DOI: 10.1126/sciimmunol.add8945
Estimating RMRs of synonymous codons from E. coli proteomic data
Authors: Mengyi Sun and Jianzhi Zhang
Date: Apr 17, 2023，Views：173
Original protocol used in research article：
Preferred synonymous codons are translated more accurately: Proteomic evidence, among-species variation, and mechanistic basis
Science Advances, Jul 6, 2022; DOI: 10.1126/sciadv.abl9812
Isolation of trigeminal neural nuclei
Authors: Minh Q Nguyen and Nicholas Ryba
Date: Apr 14, 2023，Views：184
Original protocol used in research article：
Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
eLife, Oct 8, 2019; DOI: 10.7554/eLife.49679
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