Protocols shared by authors via RaP service
Journal
Creating Arabidopsis mutants with CRISPR/Cas9 by using the plasmid pHEE401E
Authors: Upendo Lupanga and Karin Schumacher
Date: May 8, 2023，Views：490
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Study design-Senolytic doses in human livers using proof-of-concept perfusion
Authors: Stuart J. Forbes and Sofia Ferreira-Gonzalez
Date: May 8, 2023，Views：419
Original protocol used in research article：
Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage
Science Translational Medicine, Dec 7, 2022; DOI: 10.1126/scitranslmed.abj4375
MG-132/chloroquine treatment of worms
Author: hui Xiao
Date: May 7, 2023，Views：507
Original protocol used in research article：
trim-21 promotes proteasomal degradation of CED-1 for apoptotic cell clearance in C. elegans
eLife, ; DOI: 10.7554/eLife.76436
A reference phylogenetic tree and a gene presence/absence profile
Authors: Naoki Konno and Wataru Iwasaki
Date: May 5, 2023，Views：114
Original protocol used in research article：
Machine learning enables prediction of metabolic system evolution in bacteria
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.adc9130
DRG cultures
Authors: Roman J Giger and Ashley L Kalinski
Date: May 4, 2023，Views：661
Original protocol used in research article：
Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement
eLife, Dec 2, 2020; DOI: 10.7554/eLife.60223
Cell culture
Authors: Lingjun Tong, Huaxi Yi and Jiong-Wei Wang
Date: May 3, 2023，Views：748
Original protocol used in research article：
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041
Code availability
Authors: Chuan Xue and Keith J. Gooch
Date: May 3, 2023，Views：509
Original protocol used in research article：
A Mechanistic Motor-Clutch Model That Explains Cell Shape Dynamics to Cyclic Stretch
Molecular Biology of the Cell, Jan 12, 2022; DOI: 10.1091/mbc.E20-01-0087
Tissue processing for single-cell isolation
Authors: Ana J Caetano and Paul Sharpe
Date: May 3, 2023，Views：541
Original protocol used in research article：
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease
eLife, Jan 4, 2021; DOI: 10.7554/eLife.62810
Yeast agglutination assay
Authors: Sobieszczańska Beata and Dzięgiel Piotr
Date: May 2, 2023，Views：1019
Original protocol used in research article：
Norepinephrine affects the interaction of adherent-invasive Escherichia coli with intestinal epithelial cells
Virulence, Feb 4, 2021; DOI: 10.1080/21505594.2021.1882780
Preparation of monolayers before mechanical strain
Author: Blair W Benham-Pyle
Date: May 1, 2023，Views：495
Original protocol used in research article：
Increasing β-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis
eLife, Oct 26, 2016; DOI: 10.7554/eLife.19799
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