Protocols shared by authors via RaP service
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In vitro TFH stimulation assay
Authors: Phillip P. Domeier, Ziaur S.M. Rahman and Steven F. Ziegler
Date: Apr 10, 2023，Views：218
Original protocol used in research article：
B cell– and T cell–intrinsic regulation of germinal centers by thymic stromal lymphopoietin signaling
Science Immunology, Jan 6, 2023; DOI: 10.1126/sciimmunol.add9413
Genome-wide CRISPR-Cas9 screen
Authors: Zengzhang Zheng, Judy Lieberman, Xing Liu and Zengzhang Zheng
Date: Apr 6, 2023，Views：275
Original protocol used in research article：
The lysosomal Rag-Ragulator complex licenses RIPK1– and caspase-8–mediated pyroptosis by Yersinia
Science, Jun 25, 2021; DOI: 10.1126/science.abg0269
Organoid infection
Authors: Guillaume Jacquemin, Silvia Fre and Mathilde Huyghe
Date: Apr 6, 2023，Views：264
Original protocol used in research article：
Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme
eLife, ; DOI: 10.7554/eLife.76541
Generation of stable cell lines expressing 3xFLAG-CDK12
Authors: Xiangbing Qi and Ting Han
Date: Apr 5, 2023，Views：269
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Cell cluster annotation, trajectory inference, and signature enrichment
Authors: Ekaterini Maria Lyras and Alexander Mildner
Date: Apr 5, 2023，Views：307
Original protocol used in research article：
Tongue immune compartment analysis reveals spatial macrophage heterogeneity
eLife, ; DOI: 10.7554/eLife.77490
Expression and purification of CR3022 Fab and IgG
Authors: Meng Yuan, Nicholas C. Wu, Wenli Yu and Ian A. Wilson
Date: Apr 4, 2023，Views：69
Original protocol used in research article：
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV
Science, May 7, 2020; DOI: 10.1126/science.abb7269
Preparation of odorant and buffer solutions
Authors: Albert Lin, Cengiz Pehlevan, Mei Zhen and Aravinthan D.T. Samuel
Date: Apr 4, 2023，Views：321
Original protocol used in research article：
Functional imaging and quantification of multineuronal olfactory responses in C. elegans
Science Advances, Mar 1, 2023; DOI: 10.1126/sciadv.ade1249
Experimental model and subject details
Author: Lucas Pozzo-Miller
Date: Apr 4, 2023，Views：55
Original protocol used in research article：
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory
eLife, May 21, 2019; DOI: 10.7554/eLife.44182
Detection of mitochondrial ROS levels using flow cytometry
Authors: Xiaowei Zheng and Sergiu-Bogdan Catrina
Date: Apr 3, 2023，Views：424
Original protocol used in research article：
Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes
eLife, Feb 15, 2022; DOI: 10.7554/eLife.70714
Generation of rescued mouse ES cell lines expressing EGFP-tagged Suv39h proteins
Authors: Thomas Jenuwein and Kalina Malgorzata Swist-Rosowska
Date: Mar 31, 2023，Views：285
Original protocol used in research article：
Major satellite repeat RNA stabilize heterochromatin retention of Suv39h enzymes by RNA-nucleosome association and RNA:DNA hybrid formation
eLife, Aug 1, 2017; DOI: 10.7554/eLife.25293
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