Protocols shared by authors via RaP service
Journal
Isolation of peripheral blood mononuclear cells (PBMCs) from whole blood and thawing
Authors: Savannah N. Lewis and Prasanna Jagannathan
Date: Mar 14, 2023，Views：368
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Collection of human gonadal cells
Authors: Wolfram H. Gruhn, Walfred W.C. Tang and M. Azim Surani
Date: Mar 13, 2023，Views：408
Original protocol used in research article：
Epigenetic resetting in the human germ line entails histone modification remodeling
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.ade1257
Enzyme linked immunosorbant assay (ELISA)
Authors: Qiang Gao, Xiaoqin Ge, Jinxing Lu, Changgui Li, Xiangxi Wang, Weidong Yin, Yanjun Zhang and Chuan Qin
Date: Mar 10, 2023，Views：390
Original protocol used in research article：
Development of an inactivated vaccine candidate for SARS-CoV-2
Science, Jul 2, 2020; DOI: 10.1126/science.abc1932
LSK FACS-Sorting
Authors: Cristina Martínez Valiente, Cristian Garcia-Ruiz, Beatriz Rosón, Alessandro Liquori, Elisa González-Romero, Raúl Fernández-González, Isabel Gómez-Redondo, José Cervera, Alfonso Gutierrez Adan and Alejandra Sanjuan-Pla
Date: Mar 10, 2023，Views：381
Original protocol used in research article：
Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes
International Journal of Molecular Sciences, Jun 28, 2021; DOI: 10.3390/ijms22136963
Western blotting analysis
Authors: Joseph Wayne M Fowler and William C Sessa
Date: Mar 9, 2023，Views：420
Original protocol used in research article：
Inflammatory stress signaling via NF-kB alters accessible cholesterol to upregulate SREBP2 transcriptional activity in endothelial cells
eLife, Aug 12, 2022; DOI: 10.7554/eLife.79529
Lentivirus infection of organoids for inducible knockdown and activation
Author: Emma L Rawlins
Date: Mar 8, 2023，Views：1220
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
Human specimens and cells
Authors: Liam J. O’Neil, Mariana J. Kaplan and Carmelo Carmona-Rivera
Date: Mar 7, 2023，Views：366
Original protocol used in research article：
Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis
Science Advances, Oct 27, 2020; DOI: 10.1126/sciadv.abd2688
Myotube-to-macrophage conditioned medium
Authors: Nicolas J. Pillon and Juleen R. Zierath
Date: Mar 7, 2023，Views：368
Original protocol used in research article：
Distinctive exercise-induced inflammatory response and exerkine induction in skeletal muscle of people with type 2 diabetes
Science Advances, Sep 7, 2022; DOI: 10.1126/sciadv.abo3192
DNA pull-down assays
Author: Matthew W Parker
Date: Mar 6, 2023，Views：497
Original protocol used in research article：
A new class of disordered elements controls DNA replication through initiator self-assembly
eLife, Sep 27, 2019; DOI: 10.7554/eLife.48562
LipidTOX staining
Authors: Meredith H Wilson and Steven A Farber
Date: Mar 6, 2023，Views：535
Original protocol used in research article：
Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66393
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