Protocols shared by authors via RaP service
Journal
Reagents and infection with SARS-CoV-2
Authors: Gustavo Gastão Davanzo and Marcelo Hill
Date: Mar 27, 2023，Views：263
Original protocol used in research article：
PD-1/PD-L1 blockade abrogates a dysfunctional innate-adaptive immune axis in critical β-coronavirus disease
Science Advances, Sep 21, 2022; DOI: 10.1126/sciadv.abn6545
Immunofluorescence
Author: Sarah E Siegrist
Date: Mar 27, 2023，Views：296
Original protocol used in research article：
Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila
eLife, Aug 9, 2017; DOI: 10.7554/eLife.26343
Widefield and two-photon calcium imaging
Author: Matthew McGill
Date: Mar 27, 2023，Views：297
Original protocol used in research article：
Neural signatures of auditory hypersensitivity following acoustic trauma
eLife, Sep 16, 2022; DOI: 10.7554/eLife.80015
Adapted ATAC-seq protocol from Monahan et al eLife 2017
Author: Kevin Gunther Monahan
Date: Mar 27, 2023，Views：316
Original protocol used in research article：
Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons
eLife, Sep 21, 2017; DOI: 10.7554/eLife.28620
Human-induced pluripotent stem cell culture
Author: Jan M Bruder
Date: Mar 27, 2023，Views：73
Original protocol used in research article：
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids
eLife, Nov 3, 2020; DOI: 10.7554/eLife.52904
Chlorophyllase activity assay
Authors: Sitian Gu and Yuanfa Liu
Date: Mar 26, 2023，Views：269
Original protocol used in research article：
Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304
Microbial Cell Factories, Jan 12, 2021; DOI: 10.1186/s12934-020-01507-w
Data processing on the rod-shaped WSSV capsids
Authors: Yu-Zhong Zhang and Qing-Tao Shen
Date: Mar 19, 2023，Views：21
Original protocol used in research article：
Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection
Science Advances, Feb 22, 2023; DOI: 10.1126/sciadv.add2796
Auxin-inducible degradation
Authors: Mike Boxem and Jorian J Sepers
Date: Mar 17, 2023，Views：62
Original protocol used in research article：
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
eLife, Dec 10, 2020; DOI: 10.7554/eLife.62067
Plasmids for transfection
Authors: João Vasco Ferreira and Paulo Pereira
Date: Mar 16, 2023，Views：30
Original protocol used in research article：
LAMP2A regulates the loading of proteins into exosomes
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm1140
Purification, freezing, and thawing of 3D7 P. falciparum-infected red blood cells and antibody-dependent cellular cytotoxicity (ADCC ) assay
Authors: Savannah N. Lewis, Prasanna Jagannathan and Savannah Nicole Lewis
Date: Mar 14, 2023，Views：388
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
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