Protocols shared by authors via RaP service
Journal
Bacterial colonization in vivo
Authors: Kai Yang and Zhuang Liu
Date: Jul 26, 2021，Views：1008
Original protocol used in research article：
Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.aba3546
Virus-like-particle production and viral entry assays.
Authors: Shirley Qiu, Alexandra Phan, Kathy Fu and Marceline Côté
Date: Jul 23, 2021，Views：838
Original protocol used in research article：
Filoviruses Use the HOPS Complex and UVRAG To Traffic to Niemann-Pick C1 Compartments during Viral Entry
Journal of Virology, Jan 1, 2020; DOI: 10.1128/JVI.01002-20
CD107a Degranulation Assay
Authors: Sareetha Kailayangiri and Claudia Rossig
Date: Jul 19, 2021，Views：1849
Original protocol used in research article：
EZH2 Inhibition in Ewing Sarcoma Upregulates GD2 Expression for Targeting with Gene-Modified T Cells
Molecular Therapy, Feb 23, 2019; DOI: 10.1016/j.ymthe.2019.02.014
Production and titration of SARS-CoV-2 spike pseudotyped virus using lentiviral system
Authors: Jie Hu, Qing-zhu Gao, Chang-long He, Ai-long Huang, Ni Tang and Kai Wang
Date: Jul 18, 2021，Views：875
Original protocol used in research article：
Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2
Genes & Diseases, Jul 17, 2020; DOI: 10.1016/j.gendis.2020.07.006
Linear copy and split–based whole-genome amplification
Author: Chenghang Zong
Date: Jul 16, 2021，Views：873
Original protocol used in research article：
Single-cell damagenome profiling unveils vulnerable genes and functional pathways in human genome toward DNA damage
Science Advances, Jul 1, 2021; DOI: 10.1126/sciadv.abf3329
Glycolysis capability (extracellular acidification) analysis.
Authors: Qian Shen, Heather M. Evans and Chad A. Rappleye
Date: Jul 16, 2021，Views：811
Original protocol used in research article：
Metabolism of Gluconeogenic Substrates by an Intracellular Fungal Pathogen Circumvents Nutritional Limitations within Macrophages
mBio, Apr 7, 2020; DOI: 10.1128/mBio.02712-19
2.4. Vascular permeability and Evans blue dye assay
Authors: Antonio Bernad and Diego Herrero
Date: Jul 16, 2021，Views：855
Original protocol used in research article：
Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart
Redox Biology, Mar 4, 2019; DOI: 10.1016/j.redox.2019.101156
GST fusion protein pulldown assays.
Authors: Insha Mushtaq, Vimla Band and Hamid Band
Date: Jul 15, 2021，Views：942
Original protocol used in research article：
EHD1 and RUSC2 Control Basal Epidermal Growth Factor Receptor Cell Surface Expression and Recycling
Molecular and Cellular Biology, Mar 16, 2020; DOI: 10.1128/MCB.00434-19
Cell corpse assays
Authors: Mor Levi-Ferber and Sivan Henis-Korenblit
Date: Jul 15, 2021，Views：791
Original protocol used in research article：
Transdifferentiation mediated tumor suppression by the endoplasmic reticulum stress sensor IRE-1 in C. elegans
eLife, Jul 20, 2015; DOI: 10.7554/eLife.08005
Microinjections into Zebrafish Embryos
Authors: Yuanyuan Sidi and Samuel Li
Date: Jul 14, 2021，Views：944
Original protocol used in research article：
An IRAK1-PIN1 Signalling Axis Drives Intrinsic Tumour Resistance to Radiation Therapy
Nat Cell Biol, Jan 21, 2019; DOI: 10.1038/s41556-018-0260-7
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